Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40

D C Gruenert; C B Basbaum; M J Welsh; M Li; W E Finkbeiner; J A Nadel

doi:10.1073/pnas.85.16.5951

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Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40

Journal article

Open access

Peer reviewed

Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40

D C Gruenert, C B Basbaum, M J Welsh, M Li, W E Finkbeiner and J A Nadel

Proceedings of the National Academy of Sciences - PNAS, Vol.85(16), p.5951

08/1988

DOI: 10.1073/pnas.85.16.5951

PMCID: PMC281883

PMID: 2457904

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Abstract

To facilitate understanding of the mechanisms underlying pulmonary diseases, including lung cancer and cystic fibrosis, we have transformed and characterized cultures of human tracheal epithelial cells. Cells were transfected by calcium phosphate precipitation with a plasmid containing a replication-defective simian virus 40 (SV40) genome. Colonies of cells with enhanced growth potential were isolated and analyzed for transformation- and epithelial-specific characteristics. Precrisis cells were observed to express the SV40 large tumor antigen, produce cytokeratins, have microvilli, and form tight junctions. After crisis, cells continued to express the SV40 large tumor antigen as well as epithelial-specific cytokeratins and to display the apical membrane microvilli. Apical membrane Cl channels were opened in postcrisis cells exposed to 50 microM forskolin. These channels showed electrical properties similar to those observed in primary cultures. The postcrisis cells have been in culture for greater than 250 generations and are potentially "immortal." In addition to providing a useful in vitro model for the study of ion transport by human airway epithelial cells, the cells can be used to examine stages of neoplastic progression.

Oncogenes

Epithelium - pathology

Defective Viruses

Epithelium - metabolism

Humans

Ion Channels - physiology

Trachea - metabolism

Simian virus 40

Trachea - pathology

Keratins - analysis

Cell Transformation, Neoplastic

Chlorides - metabolism

Cell Line, Transformed

Cell Transformation, Viral

Details

Title: Subtitle: Characterization of human tracheal epithelial cells transformed by an origin-defective simian virus 40
Creators: D C Gruenert - Cardiovascular Research Institute, University of California, San Francisco 94143
C B Basbaum
M J Welsh
M Li
W E Finkbeiner
J A Nadel
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.85(16), p.5951
DOI: 10.1073/pnas.85.16.5951
PMID: 2457904
PMCID: PMC281883
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences; United States
Grant note: HL 29851 / NHLBI NIH HHS HL24136 / NHLBI NIH HHS
Language: English
Date published: 08/1988
Academic Unit: Neurology; Molecular Physiology and Biophysics; Neurosurgery; Internal Medicine
Record Identifier: 9984020996502771

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