Journal article
Characterization of kinetoplast DNA minicircles in Trypanosoma rangeli
Molecular and biochemical parasitology, Vol.63(1), pp.59-67
1994
DOI: 10.1016/0166-6851(94)90008-6
PMID: 8183323
Abstract
Kinetoplast DNA, the mitochondrial DNA of kinetoplastid protozoans, is a network of interlocked minicircles and maxicircles. We analyzed the sequence organization of minicircle DNAs in the E1 Tocuyo strain and the San Augustin clone B6 of
Trypanosoma rangeli. The frequencies of different minicircle types, as defined by the number of 136-bp conserved regions (CRs), are different in the two strains. About half of the 1.7-kb
T. rangeli El Tocuyo minicircles have 1 CR and most of the others have 2. In contrast, most of the 1.6-kb
T. rangeli San Augustin minicircles have 2 CRs, while some have four. The CR contains a replication origin at one end and is conserved both within and between the two strains. Comparisons of the
T. rangeli El Tocuyo and
T. rangeli San Augustin minicircle CRs with minicircle CRs of other kinetoplastid species reveal that they are most similar to those of
Trypanosoma cruzi.
Details
- Title: Subtitle
- Characterization of kinetoplast DNA minicircles in Trypanosoma rangeli
- Creators
- RenéF Recinos - University of IowaLouis V Kirchhoff - University of IowaJohn E Donelson - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.63(1), pp.59-67
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0166-6851(94)90008-6
- PMID
- 8183323
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 1994
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984280866502771
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