Characterization of macrophage polarization in periodontal disease

Carlos Garaicoa Pazmino; Tobias Fretwurst; C H Squarize; Tord Berglundh; William V Giannobile; Lena Larsson; R M Castilho

doi:10.1111/jcpe.13156

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Characterization of macrophage polarization in periodontal disease

Journal article

Peer reviewed

Characterization of macrophage polarization in periodontal disease

Carlos Garaicoa Pazmino, Tobias Fretwurst, C H Squarize, Tord Berglundh, William V Giannobile, Lena Larsson and R M Castilho

Journal of clinical periodontology, Vol.46(8), pp.830-839

08/01/2019

DOI: 10.1111/jcpe.13156

PMID: 31152604

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Abstract

Aim To explore the M1/M2 status of macrophage polarization from healthy, gingivitis, and periodontitis patient samples. Materials and methods Gingival biopsies were collected from 42 individuals (14 gingivitis, 18 periodontitis, and 10 healthy samples) receiving periodontal therapy. Histomorphology analysis was performed with haematoxylin and eosin staining. Immunofluorescence was performed using a combination of CD68 (macrophages), iNOS (M1), and CD206 (M2) in order to acquire changes in macrophage polarization at a single‐cell resolution. Macrophages were quantified under microscopy using narrow wavelength filters to detect Alexa 488, Alexa 568, Alexa 633 fluorophores, and Hoechst 33342 to identify cellular DNA content. Results Gingivitis and periodontitis samples showed higher levels of macrophages compared with healthy samples. Unexpectedly, periodontitis samples displayed lower levels of macrophages dispersed in the stromal tissues compared with gingivitis samples; however, it remained higher than healthy tissues. The polarization of macrophages appears to be reduced in periodontitis and showed similar levels to those observed in healthy tissues. Conclusions Our study found that gingivitis and periodontitis differ from each other by the levels of macrophage infiltrate, but not by changes in macrophage polarization.

Immunohistochemistry

Inflammation

allergy and immunology

gingivitis

immunologic factors

periodontitis

Details

Title: Subtitle: Characterization of macrophage polarization in periodontal disease
Creators: Carlos Garaicoa Pazmino
Tobias Fretwurst - University Medical Center Freiburg
C H Squarize - University of Michigan School of Dentistry
Tord Berglundh - University of Gothenburg
William V Giannobile - University of Michigan
Lena Larsson - University of Gothenburg
R M Castilho - University of Michigan School of Dentistry
Resource Type: Journal article
Publication Details: Journal of clinical periodontology, Vol.46(8), pp.830-839
DOI: 10.1111/jcpe.13156
PMID: 31152604
NLM abbreviation: J Clin Periodontol
ISSN: 0303-6979
eISSN: 1600-051X
Language: English
Date published: 08/01/2019
Academic Unit: Periodontics
Record Identifier: 9984130700002771

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