Characterization of monoclonal antibodies to Novikoff hepatoma cytokeratin antigen p39

Warren N Schmidt; David L. Page; Carla A Schmidt; Kathyleen B McKusick; W Steven Ward; Lubomir S Hnilica

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Characterization of monoclonal antibodies to Novikoff hepatoma cytokeratin antigen p39

Journal article

Peer reviewed

Characterization of monoclonal antibodies to Novikoff hepatoma cytokeratin antigen p39

Warren N Schmidt, David L. Page, Carla A Schmidt, Kathyleen B McKusick, W Steven Ward and Lubomir S Hnilica

Cancer research (Chicago, Ill.), Vol.44(12), pp.5867-5879

1984

PMID: 6209005

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Abstract

Three stable monoclonal antibody-producing mouse hybridoma lines have been developed which produce high-titer, immunoglobulin M antibodies specific for the Novikoff ascites hepatoma (NAH) Mr 39,000 cytokeratin antigen (p39). Immunotransfer assays of cytoskeletal protein-enriched fractions indicated p39 to be present in a range of rat tissues, including colon, breast, lung, and uterus. Two-dimensional gel immunoblots confirmed that immunoreactivity in the latter tissues was for polypeptides with similar isoelectric points to those of NAH p39; however, reactivity in the colon contained a wide range of additional isomeric forms. Immunohistochemical localization studies with these antibodies revealed enrichment of p39 in the simple epithelia or ductular structures of organs containing this antigen. In all sections examined, glandular epithelia were observed to be only weakly immunoreactive. Additional immunoblot and immunocytochemical analyses with the monoclonal antibodies and polyspecific antisera to NAH cytokeratin suggest the human Mr 40,000 cytokeratin to be similar to but not identical to NAH p39.

Tumors

Biological and medical sciences

Medical sciences

Animal tumors. Experimental tumors

Experimental digestive system and abdominal tumors

Details

Title: Subtitle: Characterization of monoclonal antibodies to Novikoff hepatoma cytokeratin antigen p39
Creators: Warren N Schmidt - Vanderbilt univ., cancer center, Nashville TN 37232, United States
David L. Page - Vanderbilt univ., cancer center, Nashville TN 37232, United States
Carla A Schmidt - Vanderbilt univ., cancer center, Nashville TN 37232, United States
Kathyleen B McKusick - Vanderbilt univ., cancer center, Nashville TN 37232, United States
W Steven Ward - Vanderbilt univ., cancer center, Nashville TN 37232, United States
Lubomir S Hnilica - Vanderbilt univ., cancer center, Nashville TN 37232, United States
Resource Type: Journal article
Publication Details: Cancer research (Chicago, Ill.), Vol.44(12), pp.5867-5879
Publisher: American Association for Cancer Research
PMID: 6209005
ISSN: 0008-5472
eISSN: 1538-7445
Language: English
Date published: 1984
Academic Unit: Gastroenterology and Hepatology; Internal Medicine
Record Identifier: 9984094592802771

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