Characterization of multiscale interactions between high intensity focused ultrasound (HIFU) and tooth dentin: the effect on matrix-metalloproteinases, bacterial biofilms and biological properties

Umer Daood; Sultan Aati; Zohaib Akram; Joyce Yee; Celine Yong; Abhishek Parolia; Liang Lin Seow; Amr S Fawzy

doi:10.1039/d1bm00555c

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Characterization of multiscale interactions between high intensity focused ultrasound (HIFU) and tooth dentin: the effect on matrix-metalloproteinases, bacterial biofilms and biological properties

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Characterization of multiscale interactions between high intensity focused ultrasound (HIFU) and tooth dentin: the effect on matrix-metalloproteinases, bacterial biofilms and biological properties

Umer Daood, Sultan Aati, Zohaib Akram, Joyce Yee, Celine Yong, Abhishek Parolia, Liang Lin Seow and Amr S Fawzy

Biomaterials science, Vol.9(15), pp.5344-5358

07/27/2021

DOI: 10.1039/d1bm00555c

PMID: 34190236

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Abstract

The aim of this study was to characterize multiscale interactions between high intensity focused ultrasound (HIFU) and dentin collagen and associated matrix-metalloproteinases, in addition to the analysis of the effect of HIFU on bacterial biofilms and biological properties. Dentin specimens were subjected to 5, 10 or 20 s HIFU. XPS spectra were acquired and TEM was performed on dentin slabs. Collagen orientation was performed using Raman spectroscopy. Calcium measurements in human dental pulpal cells (hDPCs) were carried out after 7 and 14 days. For macrophages, CD36+ and CD163+ were analysed. Biofilms were analyzed using CLSM. Tandem mass spectroscopy was performed for the detection of hydroxyproline sequences along with human MMP-2 quantification. Phosphorus, calcium, and nitrogen were detected in HIFU specimens. TEM images demonstrated the collagen network appearing to be fused together in the HIFU 10 and 20 s specimens. The band associated with 960 cm-1 corresponds to the stretching ν1 PO43-. The control specimens showed intensive calcium staining followed by HIFU 20 s > HIFU 10 s > HIFU 5 s specimens. Macrophages in the HIFU specimens co-expressed CD80+ and CD163+ cells. CLSM images showed the HIFU treatment inhibiting bacterial growth. SiteScore propensity determined the effect of HIFU on the binding site with a higher DScore representing better site exposure on MMPs. Multiscale mapping of dentin collagen after HIFU treatment showed no deleterious alterations on the organic structure of dentin.

Biofilms

Dentin

Humans

Hydroxyproline

Matrix Metalloproteinases

Tooth

Ultrasonic Waves

Details

Title: Subtitle: Characterization of multiscale interactions between high intensity focused ultrasound (HIFU) and tooth dentin: the effect on matrix-metalloproteinases, bacterial biofilms and biological properties
Creators: Umer Daood - IMU University
Sultan Aati - The University of Western Australia
Zohaib Akram - The University of Western Australia
Joyce Yee - IMU University
Celine Yong - IMU University
Abhishek Parolia - IMU University
Liang Lin Seow - University of Kuala Lumpur
Amr S Fawzy - The University of Western Australia
Resource Type: Journal article
Publication Details: Biomaterials science, Vol.9(15), pp.5344-5358
DOI: 10.1039/d1bm00555c
PMID: 34190236
NLM abbreviation: Biomater Sci
ISSN: 2047-4830
eISSN: 2047-4849
Grant note: DOI: 10.13039/501100000925, name: National Health and Medical Research Council, award: 1188401- RA/1/3330/83; DOI: 10.13039/501100013885, name: Kementerian Kesihatan Malaysia, award: FRGS/1/2020/SKK0/IMU/02/10; DOI: 10.13039/501100009381, name: International Medical University, award: BDS I-01-2020(18)
Language: English
Date published: 07/27/2021
Academic Unit: Endodontics
Record Identifier: 9984446552502771

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