Journal article
Characterization of p53 gene mutations in a Brazilian population with oral squamous cell carcinomas
International journal of oncology, Vol.24(2), pp.295-303
2004
DOI: 10.3892/ijo.24.2.295
PMID: 14719105
Abstract
Mutations in the p53 tumor suppressor gene are present in approximately 50% of all human cancers. We sought to determine the frequency and type of p53 mutations in squamous cell carcinomas (SCC) of the oral cavity in a Brazilian population. To identify p53 mutations we used PCR-SSCP in tumor tissue microdissected from paraffin- embedded and from fresh-frozen sections followed by direct sequencing of SSCP bands with altered electrophoretic mobility. We identified p53 mutations in 40% of the human SCC analyzed. The mutations were of a broad spectrum, with a preponderance of G --> A and A --> G transitions with an apparent hotspot at the CpG dinucleotide at codon 290. Patient samples were stratified according to tobacco and alcohol consumption as well as by anatomic location of the tumor, and although trends did emerge, no statistically significant associations were obtained between the occurance of TP53 mutations and these lifestyle habits. We conclude that p53 mutations are common among oral cavity cancers in this population, and stress the significance of this study since it is the first analysis of p53 mutation in oral cancer in a southern Brazilian population.
Details
- Title: Subtitle
- Characterization of p53 gene mutations in a Brazilian population with oral squamous cell carcinomas
- Creators
- Anna C. M CHAVES - Faculdade de Odontologia, BrazilKaren CHERUBINI - Faculdade de Odontologia, BrazilNilton HERTER - Departamento de Cirurgia de Cabeca e Pescoco, Porto Alegre, BrazilRoque FURIAN - Departamento de Cirurgia de Cabeca e Pescoco, Porto Alegre, BrazilDiogenes S SANTOS - Pontificia Universidade Catolica Do Rio Grande do Sul, BrazilChristopher SQUIER - College of Dentistry, Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA 52242, United StatesFrederick E DOMANN - Holden Comprehensive Cancer Center, The University of Iowa, Iowa City, IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- International journal of oncology, Vol.24(2), pp.295-303
- Publisher
- Editorial Academy of the International Journal of Oncology; Athens
- DOI
- 10.3892/ijo.24.2.295
- PMID
- 14719105
- ISSN
- 1019-6439
- eISSN
- 1791-2423
- Language
- English
- Date published
- 2004
- Academic Unit
- Oral Pathology, Radiology and Medicine; International Programs; Pathology; Surgery; Radiation Oncology
- Record Identifier
- 9984047857702771
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