Characterization of prickle isoform-specific pk pk1 and pk sple1 mutations in Drosophila melanogaster

Anthony J Lilienthal; Mrutyunjaya Parida; J Robert Manak

doi:10.17912/micropub.biology.000656

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Characterization of prickle isoform-specific pk pk1 and pk sple1 mutations in Drosophila melanogaster

Journal article

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Characterization of prickle isoform-specific pk pk1 and pk sple1 mutations in Drosophila melanogaster

Anthony J Lilienthal, Mrutyunjaya Parida and J Robert Manak

microPublication biology, Vol.2022

10/20/2022

DOI: 10.17912/micropub.biology.000656

PMCID: PMC9634456

PMID: 36338150

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Abstract

We used paired-end next generation sequencing (NGS) to characterize the classic isoform-specific pkpk1 and pksple1 mutations of the prickle gene in Drosophila melanogaster. Here we provide evidence that these previously reported null mutations are caused by either a tirant transposon insertion into the 5’ UTR of pkpk1 or a premature stop codon in the second exon of pksple1. Additional likely benign missense mutations were identified in both mutant isoforms.

Drosophila

Genotype Data

New Finding

Details

Title: Subtitle: Characterization of prickle isoform-specific pk pk1 and pk sple1 mutations in Drosophila melanogaster
Creators: Anthony J Lilienthal - University of Iowa
Mrutyunjaya Parida - University of Iowa
J Robert Manak - University of Iowa
Resource Type: Journal article
Publication Details: microPublication biology, Vol.2022
DOI: 10.17912/micropub.biology.000656
PMID: 36338150
PMCID: PMC9634456
NLM abbreviation: MicroPubl Biol
eISSN: 2578-9430
Publisher: Caltech Library
Language: English
Date published: 10/20/2022
Academic Unit: Stead Family Department of Pediatrics; Pathology; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984311560202771

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