Journal article
Characterization of regulatory (interferon-α/β) and accessory (LAF/IL 1) monokine activities from liver granuloma macrophages of Schistosoma mansoni-infected mice
The Journal of immunology (1950), Vol.138(8), pp.2653-2662
1987
DOI: 10.4049/jimmunol.138.8.2653
PMID: 3104471
Abstract
Previously it was shown that macrophages (MΦ) isolated from the vigorous (Vig) or modulated (Mod) liver granulomas (Gr) of Schistosoma mansoni-infected mice restored mitogen and parasite egg antigen-induced proliferative responses to accessory cell-depleted lymphocytes. Furthermore, supraoptimal concentrations of highly activated VigGrMΦ suppressed lymphoproliferation to a greater extent than did the lesser activated ModGrMΦ. In this study we investigated the role of soluble mediators in GrMΦ accessory/regulatory activity. Indomethacin released VigGrMΦ-mediated inhibition of mitogen but not antigen-induced lymphoproliferation. Extensively dialyzed serum-free GrMΦ culture supernatant nonspecifically suppressed SEA- or KLH-induced blastogenesis. Culture supernatants also reduced vesicular stomatitis virus-induced plaque formation in supernatant-pretreated L-929 fibroblasts. The 20 to 45 Kd GrMΦ-derived lymphoproliferation suppressive factor (SF) and the 20 to 50 Kd viral plaque-reducing factor (PRF) were stable at low pH, but became inactivated by heat and trypsin digestion. Although freshly isolated Vig or ModGrMΦ contained preformed SF and PRF, in vitro production of the factors were depressed by protein synthesis inhibitors. Moreover, SF was active only when added to cultures before day 3 of the 6-day proliferation assay. Both SF and PRF were specifically retained on rabbit anti-murine IFN-α/β immunoaffinity columns. Thus, the suppressive activity of Vig or ModGrMΦ is in part mediated by a monokine that shares physical, biological, and antigenic characteristics with murine IFN-α/β. In contrast to the suppression of antigen-driven proliferation, GrMΦ culture supernatant costimulated PHA-induced mitogenesis. The 13 to 21 Kd GrMΦ-derived lymphocyte-activating factor (LAF) was stable to heat, low pH, and trypsin digestion. Freshly isolated Vig or ModGrMΦ contained preformed LAF, although its in vitro production was depressed by protein synthesis inhibitors. The physical and biological characteristics of GrMΦ-derived LAF appear similar to IL 1. It is concluded that both Vig and ModGrMΦ secrete regulatory/accessory monokines that may contribute to the initiation and maintenance of the focal inflammatory granulomatous response.
Details
- Title: Subtitle
- Characterization of regulatory (interferon-α/β) and accessory (LAF/IL 1) monokine activities from liver granuloma macrophages of Schistosoma mansoni-infected mice
- Creators
- David E Elliott - Wayne state univ. school medicine, dep. immunology microbiology, Detroit MI 48201, United StatesVera F Righthand - Wayne state univ. school medicine, dep. immunology microbiology, Detroit MI 48201, United StatesDov L Boros - Wayne state univ. school medicine, dep. immunology microbiology, Detroit MI 48201, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.138(8), pp.2653-2662
- DOI
- 10.4049/jimmunol.138.8.2653
- PMID
- 3104471
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- American Association of Immunologists
- Language
- English
- Date published
- 1987
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094723102771
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