Journal article
Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin
Chromosoma, Vol.107(5), pp.277-285
01/01/1998
DOI: 10.1007/s004120050309
PMID: 9880760
Abstract
In a variety of organisms, euchromatic genes brought into juxtaposition with pericentric heterochromatin show position-effect variegation (PEV), a silencing of gene expression in a subset of the cells in which the gene is normally expressed. Previously, a P-element mobilization screen identified transgenic Drosophila stocks showing PEV of an hsp70-white+ reporter gene; transgenes in many of these stocks map to the chromocenter of polytene chromosome. A screen at an elevated temperature identified two stocks that under standard culture temperatures show complete repression of the hsp70-white+ transgene. The transgenes in both cases map to the chromocenter of polytene chromosomes. Different types of middle repetitive elements are adjacent to seven pericentric transgenes; unique sequences are adjacent to two of the perimetric transgenes. All of the transgenes show suppression of PEV in response to a mutation in the gene encoding heterochromatin protein 1 (HP1). This suppression correlates with a more accessible chromatin structure. The results indicate that a pericentric transgene showing PEV can be associated with different types of DNA sequences, while maintaining a common association with the chromosomal protein HP1.
Details
- Title: Subtitle
- Characterization of sequences associated with position-effect variegation at pericentric sites in Drosophila heterochromatin
- Creators
- D E Cryderman - University of IowaM H Cuaycong - Washington University in St. LouisSCR ElginL Wallrath - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Chromosoma, Vol.107(5), pp.277-285
- DOI
- 10.1007/s004120050309
- PMID
- 9880760
- NLM abbreviation
- Chromosoma
- ISSN
- 0009-5915
- eISSN
- 1432-0886
- Language
- English
- Date published
- 01/01/1998
- Academic Unit
- Biochemistry and Molecular Biology; University College Courses
- Record Identifier
- 9984288715902771
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