Characterization of structure and function of the mouse retina using pattern electroretinography, pupil light reflex, and optical coherence tomography

Kabhilan Mohan; Matthew M Harper; Helga Kecova; Eun-Ah Ye; Tatjana Lazic; Donald S Sakaguchi; Randy H Kardon; Sinisa D Grozdanic; CR: BioMed Vision Technologies, Neuroptics Inc

doi:10.1111/j.1463-5224.2012.01034.x

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Characterization of structure and function of the mouse retina using pattern electroretinography, pupil light reflex, and optical coherence tomography

Journal article

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Peer reviewed

Characterization of structure and function of the mouse retina using pattern electroretinography, pupil light reflex, and optical coherence tomography

Kabhilan Mohan, Matthew M Harper, Helga Kecova, Eun-Ah Ye, Tatjana Lazic, Donald S Sakaguchi, Randy H Kardon, Sinisa D Grozdanic and CR: BioMed Vision Technologies, Neuroptics Inc

Veterinary ophthalmology, Vol.15 Suppl 2(SUPPL.2), pp.94-104

09/2012

DOI: 10.1111/j.1463-5224.2012.01034.x

PMID: 22642927

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Abstract

To perform in vivo analysis of retinal functional and structural parameters in healthy mouse eyes. Adult C57BL/6 male mice (n = 37). Retinal function was evaluated using pattern electroretinography (pERG) and the chromatic pupil light reflex (cPLR). Structural properties of the retina and nerve fiber layer (NFL) were evaluated using spectral-domain optical coherence tomography (SD-OCT). The average pERG amplitudes were found to be 11.2 ± 0.7 μV (P50-N95, mean ± SEM), with an implicit time for P50-N95 interval of 90.4 ± 5.4 ms. Total retinal thickness was 229.5 ± 1.7 μm (mean ± SEM) in the area centralis region. The thickness of the retinal nerve fiber layer (mean ± SEM) using a circular peripapillary retinal scan centered on the optic nerve was 46.7 ± 0.9 μm (temporal), 46.1 ± 0.9 μm (superior), 45.8 ± 0.9 μm (nasal), and 48.4 ± 1 μm (inferior). The baseline pupil diameter was 2.1 ± 0.05 mm in darkness, and 1.1 ± 0.05 and 0.56 ± 0.03 mm after stimulation with red (630 nm, luminance 200 kcd/m(2)) or blue (480 nm, luminance 200 kcd/m(2)) light illumination, respectively. Pattern electroretinography, cPLR and SD-OCT analysis are reproducible techniques, which can provide important information about retinal and optic nerve function and structure in mice.

Electroretinography

Reflex, Pupillary - physiology

Retina - physiology

Optic Nerve - physiology

Animals

Mice, Inbred C57BL

Male

Mice

Tomography, Optical Coherence - methods

Retina - anatomy & histology

Details

Title: Subtitle: Characterization of structure and function of the mouse retina using pattern electroretinography, pupil light reflex, and optical coherence tomography
Creators: Kabhilan Mohan - Iowa City Department of Veterans Affairs Center for Prevention and Treatment of Vision Loss, Iowa City, IA 52246-2209, USA
Matthew M Harper
Helga Kecova
Eun-Ah Ye
Tatjana Lazic
Donald S Sakaguchi
Randy H Kardon
Sinisa D Grozdanic
CR: BioMed Vision Technologies, Neuroptics Inc
Resource Type: Journal article
Publication Details: Veterinary ophthalmology, Vol.15 Suppl 2(SUPPL.2), pp.94-104
DOI: 10.1111/j.1463-5224.2012.01034.x
PMID: 22642927
NLM abbreviation: Vet Ophthalmol
ISSN: 1463-5224
eISSN: 1463-5224
Publisher: England
Grant note: 1R01EY019294 / NEI NIH HHS
Language: English
Date published: 09/2012
Academic Unit: Iowa Neuroscience Institute; Biology; Ophthalmology and Visual Sciences
Record Identifier: 9983980050802771

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