Journal article
Characterization of the PNT1 pentamidine resistance gene of Saccharomyces cerevisiae
Antimicrobial agents and chemotherapy, Vol.38(12), pp.2850-2856
12/1994
DOI: 10.1128/AAC.38.12.2850
PMCID: PMC188296
PMID: 7695273
Abstract
The Saccharomyces cerevisiae PNT1 gene was isolated and characterized. When present in high copy number in S. cerevisiae, PNT1 confers resistance to the anti-Pneumocystis carinii drug pentamidine. The PNT1 gene encodes a previously uncharacterized polypeptide of 409 amino acids. The predicted gene product is a very basic (pI 9.9) polypeptide with one potential membrane-associated region. PNT1 is located on chromosome XVR of S. cerevisiae. It is transcribed at a very low level. Overexpression of the gene increases resistance to the cytostatic and mitochondrial DNA-damaging effects of pentamidine and related cationic compounds. Disruption of the gene leads to slightly increased levels of susceptibility to pentamidine and some related compounds.
Details
- Title: Subtitle
- Characterization of the PNT1 pentamidine resistance gene of Saccharomyces cerevisiae
- Creators
- G Ludewig - T. H. Morgan School of Biological Sciences, University of Kentucky, Lexington 40506-0225C Staben
- Resource Type
- Journal article
- Publication Details
- Antimicrobial agents and chemotherapy, Vol.38(12), pp.2850-2856
- DOI
- 10.1128/AAC.38.12.2850
- PMID
- 7695273
- PMCID
- PMC188296
- NLM abbreviation
- Antimicrob Agents Chemother
- ISSN
- 0066-4804
- eISSN
- 1098-6596
- Publisher
- United States
- Grant note
- GM46193 / NIGMS NIH HHS
- Language
- English
- Date published
- 12/1994
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9983997333902771
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