Journal article
Characterization of the functional specificity of a cloned T-cell receptor heterodimer recognizing the MART-1 melanoma antigen
Cancer research (Chicago, Ill.), Vol.55(4), pp.748-752
1995
PMID: 7531614
Abstract
T cells can play a central role in the immune response to cancer, with tumor-specific T-lymphocyte reactivity provided by the T-cell receptor (TCR) alpha and beta chain heterodimer. This study is the first report of the definitive identification and characterization of a functional tumor-associated, antigen-specific TCR by reconstitution in an alternate cell line. Jurkat T cells were transfected with the cDNAs encoding the full-length alpha and beta T-cell receptor chains from the HLA-A2 restricted, melanoma-reactive T-cell clone, clone 5. Expression of the transfected TCR was evaluated by immunofluorescence after down-modulation of the endogenous receptor with Jurkat T-cell receptor beta chain-specific mAb. Jurkat clone 5 TCR+ cells recognized MART-1 peptides presented by T2 cells in a pattern and sensitivity equivalent to native MART-1-reactive T-cells. Recognition of HLA-A2+ melanoma cell lines by the Jurkat clone 5 TCR+ cells, however, did not occur without the addition of exogenous MART-1 peptide. The cloning and expression of functional TCR genes which are capable of specifically recognizing MART-1 antigen provides reagents which could be used for the study of the mechanisms of T-cell/tumor antigen interactions and creates immortalized reagents which can facilitate studies requiring detection of the MART-1 antigen. The tumor reactivity provided by these genes could also have application in novel immunotherapeutic strategies for treating patients with melanoma, including redirection of tumor-infiltrating lymphocyte specificity and bone marrow stem cell therapy.
Details
- Title: Subtitle
- Characterization of the functional specificity of a cloned T-cell receptor heterodimer recognizing the MART-1 melanoma antigen
- Creators
- D. J Cole - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United StatesD. P Weil - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United StatesJ Shilyansky - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United StatesM Custer - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United StatesY Kawakami - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United StatesS. A Rosenberg - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United StatesM. I Nishimura - NIH, national cancer inst., surgery branch, Bethesda MD 20892, United States
- Resource Type
- Journal article
- Publication Details
- Cancer research (Chicago, Ill.), Vol.55(4), pp.748-752
- Publisher
- American Association for Cancer Research
- PMID
- 7531614
- ISSN
- 0008-5472
- eISSN
- 1538-7445
- Language
- English
- Date published
- 1995
- Academic Unit
- Stead Family Department of Pediatrics; Surgery
- Record Identifier
- 9984321862002771
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