Journal article
Characterization of the most prevalent colonization factor antigens present in Chilean clinical enterotoxigenic Escherichia coli strains using a new multiplex polymerase chain reaction
Diagnostic microbiology and infectious disease, Vol.65(3), pp.217-223
2009
DOI: 10.1016/j.diagmicrobio.2009.07.005
PMID: 19733027
Abstract
Current methods to detect the colonization factor antigens (CFAs) associated with enterotoxigenic
Escherichia coli (ETEC) strains are cumbersome, with some methods requiring antibodies that are not readily available. To achieve a gene-based method, we designed 2 multiplex polymerase chain reaction reactions to detect genes encoding the most common ETEC fimbrial colonization factors, including CFA/I and coli surface (CS) antigens CS1, CS2, CS3, CS4, CS5, and CS6. Analysis of 183 clinical ETEC strains shows that the most prevalent colonization factors were CFA/I only, CS1 and CS3, CS2 and CS3, and CS6 only. Interestingly, we identified 3 clinical isolates expressing CS1 only without its regulator
rns. The method described here proved to be rapid and robust and correlates well with phenotypic expression of the CFAs, becoming a novel molecular diagnostic and research tool for future epidemiologic studies.
Details
- Title: Subtitle
- Characterization of the most prevalent colonization factor antigens present in Chilean clinical enterotoxigenic Escherichia coli strains using a new multiplex polymerase chain reaction
- Creators
- Roberto M Vidal - Faculty of Medicine, University of Chile, Santiago, 8389100 ChilePatricio Valenzuela - Faculty of Medicine, University of Chile, Santiago, 8389100 ChileKelly Baker - Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USARosanna Lagos - Centro para Vacunas en Desarrollo, Santiago, ChileMario Esparza - Faculty of Medicine, University of Chile, Santiago, 8389100 ChileSofie Livio - Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USAMauricio Farfán - Faculty of Medicine, University of Chile, Santiago, 8389100 ChileJames P Nataro - Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USAMyron M Levine - Center for Vaccine Development, University of Maryland, Baltimore, MD 21201, USAValeria Prado - Faculty of Medicine, University of Chile, Santiago, 8389100 Chile
- Resource Type
- Journal article
- Publication Details
- Diagnostic microbiology and infectious disease, Vol.65(3), pp.217-223
- DOI
- 10.1016/j.diagmicrobio.2009.07.005
- PMID
- 19733027
- NLM abbreviation
- Diagn Microbiol Infect Dis
- ISSN
- 0732-8893
- eISSN
- 1879-0070
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 2009
- Academic Unit
- Occupational and Environmental Health; Epidemiology
- Record Identifier
- 9984214681302771
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