Journal article
Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy mouse eyes
Current eye research, Vol.26(6), pp.371-378
06/2003
DOI: 10.1076/ceyr.26.5.371.15439
PMID: 12868018
Abstract
To characterize the pupil light reflex (PLR), electroretinographic (ERG) and tonometric parameters which might be of importance for the in vivo characterization of mouse models of chronic ocular hypertension. C57/BL6 mice were used for experiments. The PLR was evaluated with a computerized pupillometer (n = 14), ERGs were recorded simultaneously from both eyes (n = 23) and IOP was measured with a modified Goldmann tonometer (n = 23). The analysis of the PLR parameters confirmed the consensual PLR did not have significantly different amplitude (p > 0.1) and latency time (p > 0.1) compared to the direct PLR. However, PLR velocity (p = 0.004) was significantly smaller in the consensual PLR. Electroretinography revealed a-wave amplitude of 168.3 +/- 9.6 microV with latency of 27.5 +/- 0.6 ms and b-wave 403 +/- 28.8 microV with latency of 22.7 +/- 0.6 ms. The flicker ERG recording revealed amplitudes of 20.6 +/- 2.4 microV. Tonometry experiments revealed that modified Goldmann tonometer measurements correlated well with invasive manometry (r(2) = 0.89). The mean IOP of the mouse was 15.3 +/- 0.6 mmHg. Consensual PLR in mice is relatively slower than the direct PLR, but retains the same degree of constriction comparing to the direct PLR. A modified Goldmann tonometer seems to be a reliable non-invasive tool for IOP measurements in mice.
Details
- Title: Subtitle
- Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy mouse eyes
- Creators
- Sinisa Grozdanic - Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. sgrozdan@iastate.eduDaniel M BettsRachell A AllbaughDonald S SakaguchiYoung H KwonRandy H KardonIoana M Sonea
- Resource Type
- Journal article
- Publication Details
- Current eye research, Vol.26(6), pp.371-378
- DOI
- 10.1076/ceyr.26.5.371.15439
- PMID
- 12868018
- NLM abbreviation
- Curr Eye Res
- ISSN
- 0271-3683
- eISSN
- 1460-2202
- Publisher
- England
- Language
- English
- Date published
- 06/2003
- Academic Unit
- Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
- Record Identifier
- 9983979940402771
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