Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy rat eyes

Sinisa Grozdanic; Donald S Sakaguchi; Young H Kwon; Randy H Kardon; Ioana M Sonea

doi:10.1076/ceyr.25.2.69.10156

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Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy rat eyes

Journal article

Peer reviewed

Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy rat eyes

Sinisa Grozdanic, Donald S Sakaguchi, Young H Kwon, Randy H Kardon and Ioana M Sonea

Current eye research, Vol.25(2), pp.69-78

08/2002

DOI: 10.1076/ceyr.25.2.69.10156

PMID: 12525960

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Abstract

To characterize the pupil light reflex (PLR), electroretinographic (ERG) and tonometric parameters in healthy rat eyes. Brown Norway rats were used for experiments. The PLR was evaluated with a computerized pupillometer (n = 27), ERGs were recorded simultaneously from both eyes (n = 27) and IOP was measured with a Tonopen (n = 15). The analysis of the PLR parameters confirmed that the consensual PLR had a significantly smaller amplitude (0.1-0.2 mm; p = 0.03) and an increased latency time (p = 0.001) compared to the direct PLR. Electroretinography revealed an a-wave amplitude of 207.2 +/- 13 microV with a latency of 25.6 +/- 0.7 ms, and a b-wave 554.3 +/- 24.5 microV with a latency of 21.4 + 1.8 ms. The flicker ERG recording revealed amplitudes of 40.6 +/- 2.4 microV. Tonometry measurements revealed that isoflurane, but not halothane, anesthesia suppressed the IOP (non-anesthetized: 25.3 +/- 1.0 mmHg; 1% halothane + 30% NO: 26.2 +/- 1.1 (p > 0.1); 1% isoflurane + 30% NO: 20.1 + 1.6 (p < 0.05)). Consensual PLR in rats has a relative deficit compared to the direct PLR. Isoflurane anesthesia has a suppressive effect on the IOP in healthy rat eyes.

Electroretinography

Intraocular Pressure - drug effects

Anesthetics, Inhalation - administration & dosage

Isoflurane - administration & dosage

Intraocular Pressure - physiology

Rats

Halothane - administration & dosage

Reflex, Pupillary - physiology

Retina - physiology

Rats, Inbred BN

Animals

Light

Tonometry, Ocular

Models, Animal

Details

Title: Subtitle: Characterization of the pupil light reflex, electroretinogram and tonometric parameters in healthy rat eyes
Creators: Sinisa Grozdanic - Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, IA 50011, USA. sgrozdan@iastate.edu
Donald S Sakaguchi
Young H Kwon
Randy H Kardon
Ioana M Sonea
Resource Type: Journal article
Publication Details: Current eye research, Vol.25(2), pp.69-78
DOI: 10.1076/ceyr.25.2.69.10156
PMID: 12525960
NLM abbreviation: Curr Eye Res
ISSN: 0271-3683
eISSN: 1460-2202
Publisher: England
Language: English
Date published: 08/2002
Academic Unit: Iowa Neuroscience Institute; Ophthalmology and Visual Sciences
Record Identifier: 9983979945702771

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