Journal article
Characterization of the purified N-type Ca2+ channel and the cation sensitivity of ω-conotoxin GVIA binding
Neuropharmacology, Vol.32(11), pp.1127-1139
11/1993
DOI: 10.1016/0028-3908(93)90007-P
PMID: 8107967
Abstract
A functional N-type Ca2+ channel (omega-conotoxin GVIA receptor) has been purified from rabbit brain and shown to be composed of four subunits of molecular weights 230 K (alpha 1B), 160 K (alpha 2 delta), 95 K and 57 K (beta 3) [Witcher D. R., De Waard M., Sakamoto J., Franzini-Armstrong C., Pragnell M., Kahl S.D. and Campbell K. D. (1993) Science 261: 486-489]. These four subunits migrate on sucrose density gradients as a single complex and are identified by subunit specific polyclonal antibodies. Polyclonal antibodies against the purified receptor complex immunoprecipitate greater than 90% of the [125I]omega-conotoxin GVIA (omega-CgTx) binding sites in solubilized crude rabbit brain membranes. Furthermore, polyclonal antibodies affinity-purified against unique GST fusion proteins from two of the cloned subunits in the complex (alpha 1B and beta 3) specifically immunoprecipitated [125I]omega-CgTx binding sites and not [3H]PN200-110 binding sites. Analysis of [125I]omega-CgTx binding to the purified N-type Ca2+ channel demonstrated that the equilibrium binding was sensitive to increasing cation concentration. The IC50 for calcium and barium was 2.5 and 5 mM, respectively. [125I]omega-CgTx binding was not significantly reduced within 15 min after the addition of 50 mM barium. However, single channel analysis of the purified N-type Ca2+ channel preincubated with 10 microM omega-CgTx demonstrated that in the presence of 50 mM barium and 0.5 microM omega-CgTx, channel activity was detected but at a low open state probability (P < 0.10). These data suggest that the Ca2+ binding site(s) allosterically regulates the omega-CgTx binding site. Since the channel gating persisted in the presence of omega-CgTx, the omega-CgTx binding site may not be located within the pore of the channel and may be different from intra-pore Ca2+ binding sites.
Details
- Title: Subtitle
- Characterization of the purified N-type Ca2+ channel and the cation sensitivity of ω-conotoxin GVIA binding
- Creators
- Derrick R WitcherMichel De WaardKevin P Campbell
- Resource Type
- Journal article
- Publication Details
- Neuropharmacology, Vol.32(11), pp.1127-1139
- DOI
- 10.1016/0028-3908(93)90007-P
- PMID
- 8107967
- ISSN
- 0028-3908
- eISSN
- 1873-7064
- Language
- English
- Date published
- 11/1993
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068372502771
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