Characterization of the receptor-binding domain of Ebola glycoprotein in viral entry

Jizhen Wang; Balaji Manicassamy; Michael Caffrey; Lijun Rong

doi:10.1007/s12250-011-3194-9

Back

Characterization of the receptor-binding domain of Ebola glycoprotein in viral entry

Journal article

Open access

Peer reviewed

Characterization of the receptor-binding domain of Ebola glycoprotein in viral entry

Jizhen Wang, Balaji Manicassamy, Michael Caffrey and Lijun Rong

Virologica Sinica, Vol.26(3), pp.156-170

06/2011

DOI: 10.1007/s12250-011-3194-9

PMCID: PMC7091247

PMID: 21667336

Files and links (1)

url

https://doi.org/10.1007/s12250-011-3194-9View

Published (Version of record) Open Access

Abstract

Ebola virus infection causes severe hemorrhagic fever in human and non-human primates with high mortality. Viral entry/infection is initiated by binding of glycoprotein GP protein on Ebola virion to host cells, followed by fusion of virus-cell membrane also mediated by GP. Using an human immunodeficiency virus (HIV)-based pseudotyping system, the roles of 41 Ebola GP1 residues in the receptor-binding domain in viral entry were studied by alanine scanning substitutions. We identified that four residues appear to be involved in protein folding/structure and four residues are important for viral entry. An improved entry interference assay was developed and used to study the role of these residues that are important for viral entry. It was found that R64 and K95 are involved in receptor binding. In contrast, some residues such as I170 are important for viral entry, but do not play a major role in receptor binding as indicated by entry interference assay and/or protein binding data, suggesting that these residues are involved in post-binding steps of viral entry. Furthermore, our results also suggested that Ebola and Marburg viruses share a common cellular molecule for entry.

Protein Structure, Tertiary

Amino Acid Sequence

Cell Line

Viral Envelope Proteins - genetics

Receptors, Virus - metabolism

Humans

Molecular Sequence Data

Ebolavirus - genetics

Virus Internalization

Amino Acid Motifs

Ebolavirus - chemistry

Hemorrhagic Fever, Ebola - virology

Viral Envelope Proteins - chemistry

Viral Envelope Proteins - metabolism

Protein Binding

Ebolavirus - physiology

Hemorrhagic Fever, Ebola - metabolism

Details

Title: Subtitle: Characterization of the receptor-binding domain of Ebola glycoprotein in viral entry
Creators: Jizhen Wang - Department of Microbiology and Immunology, College of Medicine, University of Illinois at Chicago, USA
Balaji Manicassamy
Michael Caffrey
Lijun Rong
Resource Type: Journal article
Publication Details: Virologica Sinica, Vol.26(3), pp.156-170
DOI: 10.1007/s12250-011-3194-9
PMID: 21667336
PMCID: PMC7091247
ISSN: 1674-0769
eISSN: 1995-820X
Grant note: R01 AI059570 / NIAID NIH HHS AI059570 / NIAID NIH HHS U01 AI077767 / NIAID NIH HHS AI077767 / NIAID NIH HHS
Language: English
Date published: 06/2011
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083876702771

Metrics

17 Record Views