Characterization of transgenic mice expressing cancer-associated variants of human NOTCH1

Katherine E Berquam-Vrieze; Deborah A Swing; Lino Tessarollo; Adam J Dupuy

doi:10.1002/dvg.20798

Back

Characterization of transgenic mice expressing cancer-associated variants of human NOTCH1

Journal article

Open access

Characterization of transgenic mice expressing cancer-associated variants of human NOTCH1

Katherine E Berquam-Vrieze, Deborah A Swing, Lino Tessarollo and Adam J Dupuy

Genesis (New York, N.Y. : 2000), Vol.50(2), pp.112-118

02/2012

DOI: 10.1002/dvg.20798

PMCID: PMC3682665

PMID: 21898766

Files and links (1)

url

https://doi.org/10.1002/dvg.20798View

Published (Version of record) Open Access

Abstract

The Notch1 receptor plays a critical role in cell fate decisions during development. Activation of Notch signaling has been implicated in several types of cancer, particularly T-cell acute lymphoblastic leukemia (T-ALL). Consequently, several transgenic mouse strains have been made to study the role of Notch1 in T-ALL. However, the existing Notch1 transgenic lines mimic a translocation event found in only ~1% of T-ALL cases. Here we describe three novel NOTCH1 transgenic mouse strains that have Cre-inducible expression of the entire human NOTCH1 locus, each possessing a common mutation found in T-ALL. Unlike existing Notch1 transgenic strains, these NOTCH1 transgenic strains express full-length receptors from an endogenous human promoter that should be susceptible to a number of Notch antagonists that have recently been developed. These strains will allow researchers to modulate Notch signaling to study both normal development and cancer biology.

mouse models of cancer

BAC transgenesis

BAC recombineering

Details

Title: Subtitle: Characterization of transgenic mice expressing cancer-associated variants of human NOTCH1
Creators: Katherine E Berquam-Vrieze - Department of Anatomy & Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242
Deborah A Swing - Mouse Cancer Genetics Program, National Cancer Institute, Frederick, Maryland 21701
Lino Tessarollo - Mouse Cancer Genetics Program, National Cancer Institute, Frederick, Maryland 21701
Adam J Dupuy - Department of Anatomy & Cell Biology, Carver College of Medicine, University of Iowa, Iowa City, Iowa 52242
Resource Type: Journal article
Publication Details: Genesis (New York, N.Y. : 2000), Vol.50(2), pp.112-118
DOI: 10.1002/dvg.20798
PMID: 21898766
PMCID: PMC3682665
ISSN: 1526-954X
eISSN: 1526-968X
Grant note: R01 CA130867-04 || CA / National Cancer Institute : NCI
Language: English
Date published: 02/2012
Academic Unit: Anatomy and Cell Biology; Pathology
Record Identifier: 9984025444802771

Metrics

20 Record Views

5 Times Cited - Web of Science