Characterizing harmful advanced glycation end-products (AGEs) and ribosylated aggregates of yellow mustard seed phytocystatin: Effects of different monosaccharides

Azaj Ahmed; Anas Shamsi; Bilqees Bano

doi:10.1016/j.saa.2016.08.004

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Characterizing harmful advanced glycation end-products (AGEs) and ribosylated aggregates of yellow mustard seed phytocystatin: Effects of different monosaccharides

Journal article

Peer reviewed

Characterizing harmful advanced glycation end-products (AGEs) and ribosylated aggregates of yellow mustard seed phytocystatin: Effects of different monosaccharides

Azaj Ahmed, Anas Shamsi and Bilqees Bano

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Vol.171, pp.183-192

01/15/2017

DOI: 10.1016/j.saa.2016.08.004

PMID: 27526342

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Abstract

Advanced glycation end products (AGEs) are at the core of variety of diseases ranging from diabetes to renal failure and hence gaining wide consideration. This study was aimed at characterizing the AGEs of phytocystatin isolated from mustard seeds (YMP) when incubated with different monosaccharides (glucose, ribose and mannose) using fluorescence, ultraviolet, circular dichroism (CD) spectroscopy and microscopy. Ribose was found to be the most potent glycating agent as evident by AGEs specific fluorescence and absorbance. YMP exists as a molten globule like structure on day 24 as depicted by high ANS fluorescence and altered intrinsic fluorescence. Glycated YMP as AGEs and ribose induced aggregates were observed at day 28 and 32 respectively. In our study we have also examined the anti-aggregative potential of polyphenol, resveratrol. Our results suggested the anti-aggregative behavior of resveratrol as it prevented the in vitro aggregation of YMP, although further studies are required to decode the mechanism by which resveratrol prevents the aggregation.

Circular Dichroism

Comet Assay

Congo Red - chemistry

Cystatins - chemistry

Cystatins - ultrastructure

Glycation End Products, Advanced - chemistry

Glycosylation

Monosaccharides - chemistry

Mustard Plant - chemistry

Nephelometry and Turbidimetry

Papain - antagonists & inhibitors

Protein Aggregates

Protein Structure, Secondary

Protein Structure, Tertiary

Ribose - chemistry

Seeds - chemistry

Spectrometry, Fluorescence

Spectrophotometry, Ultraviolet

Thiazoles - chemistry

Details

Title: Subtitle: Characterizing harmful advanced glycation end-products (AGEs) and ribosylated aggregates of yellow mustard seed phytocystatin: Effects of different monosaccharides
Creators: Azaj Ahmed - Aligarh Muslim University
Anas Shamsi - Aligarh Muslim University
Bilqees Bano - Aligarh Muslim University
Resource Type: Journal article
Publication Details: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, Vol.171, pp.183-192
DOI: 10.1016/j.saa.2016.08.004
PMID: 27526342
ISSN: 1386-1425
eISSN: 1873-3557
Language: English
Date published: 01/15/2017
Academic Unit: Internal Medicine
Record Identifier: 9985177943902771

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