Characterizing the antitumor response in mice treated with antigen-loaded polyanhydride microparticles

Vijaya B Joshi; Sean M Geary; Brenda R Carrillo-Conde; Balaji Narasimhan; Aliasger K Salem

doi:10.1016/j.actbio.2012.11.001

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Characterizing the antitumor response in mice treated with antigen-loaded polyanhydride microparticles

Journal article

Peer reviewed

Characterizing the antitumor response in mice treated with antigen-loaded polyanhydride microparticles

Vijaya B Joshi, Sean M Geary, Brenda R Carrillo-Conde, Balaji Narasimhan and Aliasger K Salem

Acta biomaterialia, Vol.9(3), pp.5583-5589

03/2013

DOI: 10.1016/j.actbio.2012.11.001

PMID: 23153760

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https://www.ncbi.nlm.nih.gov/pmc/articles/3562412View

Open Access

Abstract

Delivery of vaccine antigens with an appropriate adjuvant can trigger potential immune responses against cancer leading to reduced tumor growth and improved survival. In this study, various formulations of a bioerodible amphiphilic polyanhydride copolymer based on 1,8-bis(p-carboxyphenoxy)-3,6-dioxaoctane (CPTEG) and 1,6-bis(p-carboxyphenoxy) hexane (CPH) with inherent adjuvant properties were evaluated for antigen-loading properties, immunogenicity and antitumor activity. Mice were vaccinated with 50:50 CPTEG:CPH microparticles encapsulating a model tumor antigen, ovalbumin (OVA), in combination with the Toll-like receptor-9 agonist, CpG oligonucleotide 1826 (CpG ODN). Mice treated with OVA-encapsulated CPTEG:CPH particles elicited the highest CD8+ T cell responses on days 14 and 20 when compared to other treatment groups. This treatment group also displayed the most delayed tumor progression and the most extended survival times. Particles encapsulating OVA and CpG ODN generated the highest anti-OVA IgG1 antibody responses in mice but these mice did not show significant tumor protection. These results suggest that antigen-loaded CPTEG:CPH microparticles can stimulate antigen-specific cellular responses and could therefore potentially be used to promote antitumor responses in cancer patients.

Antigen

Polyanhydride

Antitumor immune response

Biodegradable polymer

Microparticles

Details

Title: Subtitle: Characterizing the antitumor response in mice treated with antigen-loaded polyanhydride microparticles
Creators: Vijaya B Joshi - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA
Sean M Geary - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA
Brenda R Carrillo-Conde - Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA
Balaji Narasimhan - Department of Chemical and Biological Engineering, Iowa State University, Ames, IA 50011, USA
Aliasger K Salem - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA
Resource Type: Journal article
Publication Details: Acta biomaterialia, Vol.9(3), pp.5583-5589
DOI: 10.1016/j.actbio.2012.11.001
PMID: 23153760
NLM abbreviation: Acta Biomater
ISSN: 1742-7061
eISSN: 1878-7568
Publisher: Elsevier Ltd
Language: English
Date published: 03/2013
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering
Record Identifier: 9983985973302771

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