Journal article
Charge–charge interactions are the primary determinants of the p K values of the ionizable groups in Ribonuclease T1
Biophysical chemistry, Vol.101, pp.211-219
2002
DOI: 10.1016/S0301-4622(02)00192-8
Abstract
Coulomb's law and a finite difference Poisson–Boltzmann based analysis are used to predict the p
K values for 15 ionizable side chains (6 Asp, 6 Glu and 3 His) in ribonuclease T1. These predicted values are compared to the measured p
K values to gain insight into the most important factors that influence the p
K values of the ionizable groups in proteins. Charge–charge interactions are clearly the most important factor that determines the p
K values of most ionizable groups in ribonuclease T1. However, p
K values can be shifted by several p
K units by the Born self energy associated with burying ionizable groups and by favorable intramolecular hydrogen bonding.
Details
- Title: Subtitle
- Charge–charge interactions are the primary determinants of the p K values of the ionizable groups in Ribonuclease T1
- Creators
- C.Nick Pace - Texas A&M UniversityBeatrice M.P Huyghues-Despointes - Texas A&M UniversityJames M Briggs - University of HoustonGerald R Grimsley - Texas A&M UniversityJ.Martin Scholtz - Texas A&M University
- Resource Type
- Journal article
- Publication Details
- Biophysical chemistry, Vol.101, pp.211-219
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0301-4622(02)00192-8
- ISSN
- 0301-4622
- eISSN
- 1873-4200
- Language
- English
- Date published
- 2002
- Academic Unit
- Research Administration; Pharmaceutical Sciences and Experimental Therapeutics; Biochemistry and Molecular Biology; Chemistry
- Record Identifier
- 9984288726702771
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