Logo image
Back
Chelating agents inhibit activity and prevent expression of streptococcal glucan-binding lectins
Journal article   Open access   Peer reviewed

Chelating agents inhibit activity and prevent expression of streptococcal glucan-binding lectins

Lü-Lü, J S Singh, M Y Galperin, D Drake, K G Taylor and R J Doyle
Infection and immunity, Vol.60(9), pp.3807-3813
09/1992
DOI: 10.1128/iai.60.9.3807-3813.1992
PMCID: PMC257393
PMID: 1500189
url
https://europepmc.org/articles/pmc257393View
Published (Version of record) Open Access

Abstract

Several of the cariogenic mutans streptococci produce cell wall-associated glucan-binding lectins (GBLs). The lectins bind alpha-1,6-linked glucans and have no affinity for other polysaccharides or anomeric linkages. When citrate or lactate was included in the growth medium, expression of the activities of the GBLs of Streptococcus cricetus and S. sobrinus was prevented. Furthermore, chelating agents, including citrate, lactate, EDTA, and acetylacetone, were able to reversibly inhibit glucan-induced aggregation of GBL+ streptococci. In addition, the chelating agents prevented sucrose-dependent streptococcal adhesion to glass surfaces and dispersed preformed adherent masses of the streptococci. Neither citrate nor other chelating agents modified the activities of glucosyltransferases. Expression of the lectin could only be achieved by the addition of manganous ion to the growth medium. Chloramphenicol and other metabolic inhibitors prevented synthesis of GBL in cells obtained from manganese-deficient medium and shifted to manganous ion-sufficient medium. The GBL may be a manganoprotein, the manganese of which may be perturbed, but not removed, by chelating agents. During synthesis of the GBL, manganous ion may be required in order for the protein to achieve an active conformation. Citrate or other chelating agents may have promise as anticaries agents.

Details

Metrics

13 Record Views
12 Times Cited - Web of Science
Logo image