Journal article
Chemical and Genetic Engineering of Selective Ion Channel-Ligand Interactions
Science (American Association for the Advancement of Science), Vol.333(6047), pp.1292-1296
2011
DOI: 10.1126/science.1206606
PMID: 21885782
Abstract
Ionic flux mediates essential physiological and behavioral functions in defined cell populations. Cell type-specific activators of diverse ionic conductances are needed for probing these effects. We combined chemistry and protein engineering to enable the systematic creation of a toolbox of ligand-gated ion channels (LGICs) with orthogonal pharmacologic selectivity and divergent functional properties. The LGICs and their small-molecule effectors were able to activate a range of ionic conductances in genetically specified cell types. LGICs constructed for neuronal perturbation could be used to selectively manipulate neuron activity in mammalian brains in vivo. The diversity of ion channel tools accessible from this approach will be useful for examining the relationship between neuronal activity and animal behavior, as well as for cell biological and physiological applications requiring chemical control of ion conductance.
Details
- Title: Subtitle
- Chemical and Genetic Engineering of Selective Ion Channel-Ligand Interactions
- Creators
- Christopher J MAGNUS - Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United StatesPeter H LEE - Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United StatesDeniz ATASOY - Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United StatesHelen H SU - Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United StatesLoren L LOOGER - Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United StatesScott M STERNSON - Janelia Farm Research Campus, Howard Hughes Medical Institute, 19700 Helix Drive, Ashburn, VA 20147, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.333(6047), pp.1292-1296
- DOI
- 10.1126/science.1206606
- PMID
- 21885782
- NLM abbreviation
- Science
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Publisher
- American Association for the Advancement of Science; Washington, DC
- Language
- English
- Date published
- 2011
- Academic Unit
- Iowa Neuroscience Institute; Fraternal Order of Eagles Diabetes Research Center; Neuroscience and Pharmacology
- Record Identifier
- 9984040286302771
Metrics
46 Record Views