Chemosensory regulation of a HEAT-repeat protein couples aggregation and sporulation in Myxococcus xanthus

Cynthia L Darnell; Janet M Wilson; Nitija Tiwari; Ernesto J Fuentes; John R Kirby

doi:10.1128/JB.01866-14

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Chemosensory regulation of a HEAT-repeat protein couples aggregation and sporulation in Myxococcus xanthus

Journal article

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Chemosensory regulation of a HEAT-repeat protein couples aggregation and sporulation in Myxococcus xanthus

Cynthia L Darnell, Janet M Wilson, Nitija Tiwari, Ernesto J Fuentes and John R Kirby

Journal of bacteriology, Vol.196(17), pp.3160-3168

09/2014

DOI: 10.1128/JB.01866-14

PMCID: PMC4135651

PMID: 24957622

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Abstract

Chemosensory systems are complex, highly modified two-component systems (TCS) used by bacteria to control various biological functions ranging from motility to sporulation. Chemosensory systems and TCS both modulate phosphorelays comprised of histidine kinases and response regulators, some of which are single-domain response regulators (SD-RRs) such as CheY. In this study, we have identified and characterized the Che7 chemosensory system of Myxococcus xanthus, a common soil bacterium which displays multicellular development in response to stress. Both genetic and biochemical analyses indicate that the Che7 system regulates development via a direct interaction between the SD-RR CheY7 and a HEAT repeat domain-containing protein, Cpc7. Phosphorylation of the SD-RR affects the interaction with its target, and residues within the α4-β5-α5 fold of the REC domain govern this interaction. The identification of the Cpc7 interaction with CheY7 extends the diversity of known targets for SD-RRs in biological systems.

Amino Acid Sequence

Multigene Family

Bacterial Proteins - genetics

Myxococcus xanthus - genetics

Molecular Sequence Data

Myxococcus xanthus - metabolism

Phylogeny

Gene Expression Regulation, Bacterial - physiology

Spores, Bacterial - genetics

Spores, Bacterial - physiology

Myxococcus xanthus - physiology

Protein Binding

Bacterial Proteins - metabolism

Protein Conformation

Details

Title: Subtitle: Chemosensory regulation of a HEAT-repeat protein couples aggregation and sporulation in Myxococcus xanthus
Creators: Cynthia L Darnell - Department of Microbiology, University of Iowa, Iowa City, Iowa, USA
Janet M Wilson - Division of Select Agents and Toxins, Office of Public Health Preparedness and Response, Centers for Disease Control and Prevention, Atlanta, Georgia, USA
Nitija Tiwari - Department of Biochemistry, University of Iowa, Iowa City, Iowa, USA
Ernesto J Fuentes - Department of Biochemistry, University of Iowa, Iowa City, Iowa, USA
John R Kirby - Department of Microbiology, University of Iowa, Iowa City, Iowa, USA john-kirby@uiowa.edu
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.196(17), pp.3160-3168
Publisher: United States
DOI: 10.1128/JB.01866-14
PMID: 24957622
PMCID: PMC4135651
ISSN: 0021-9193
eISSN: 1098-5530
Grant note: T32 GM008365 / NIGMS NIH HHS
Language: English
Date published: 09/2014
Academic Unit: Biochemistry and Molecular Biology
Record Identifier: 9984024517502771

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