Chick hair cells do not exhibit voltage‐dependent somatic motility

David Z. Z He; Kirk W Beisel; Lin Chen; Da‐Lian Ding; Shuping Jia; Bernd Fritzsch; Richard Salvi

doi:10.1113/jphysiol.2002.026070

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Chick hair cells do not exhibit voltage‐dependent somatic motility

Journal article

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Chick hair cells do not exhibit voltage‐dependent somatic motility

David Z. Z He, Kirk W Beisel, Lin Chen, Da‐Lian Ding, Shuping Jia, Bernd Fritzsch and Richard Salvi

The Journal of physiology, Vol.546(2), pp.511-520

01/2003

DOI: 10.1113/jphysiol.2002.026070

PMCID: PMC2342527

PMID: 12527737

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Abstract

It is generally believed that mechanical amplification by cochlear hair cells is necessary to enhance the sensitivity and frequency selectivity of hearing. In the mammalian ear, the basis of cochlear amplification is believed to be the voltage-dependent electromotility of outer hair cells (OHCs). The avian basilar papilla contains tall and short hair cells, with the former being comparable to inner hair cells, and the latter comparable to OHCs, based on their innervation patterns. In this study, we sought evidence for somatic electromotility by direct measurements of voltage-dependent length changes in both tall and short hair cells at nanometre resolution. Microchamber and whole-cell voltage-clamp techniques were used. Motility was measured with a photodiode-based measurement system. Non-linear capacitance, an electrical signature of somatic motility, was also measured to complement motility measurement. Significantly, chick hair cells did not exhibit somatic motility nor express non-linear capacitance. The lack of somatic motility suggests that in avian hair cells the active process resides elsewhere, most likely in the hair cell stereocilia.

Details

Title: Subtitle: Chick hair cells do not exhibit voltage‐dependent somatic motility
Creators: David Z. Z He - Hair Cell Biophysics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA
Kirk W Beisel - Department of Genetics, Boys Town National Research Hospital, Omaha, NE, USA
Lin Chen - Hearing Research Laboratory, University of Buffalo, NY, USA
Da‐Lian Ding - Hearing Research Laboratory, University of Buffalo, NY, USA
Shuping Jia - Hair Cell Biophysics Laboratory, Boys Town National Research Hospital, Omaha, NE, USA
Bernd Fritzsch - Department of Biomedical Sciences, Creighton University, Omaha, NE, USA
Richard Salvi - Hearing Research Laboratory, University of Buffalo, NY, USA
Resource Type: Journal article
Publication Details: The Journal of physiology, Vol.546(2), pp.511-520
DOI: 10.1113/jphysiol.2002.026070
PMID: 12527737
PMCID: PMC2342527
NLM abbreviation: J Physiol
ISSN: 0022-3751
eISSN: 1469-7793
Language: English
Date published: 01/2003
Academic Unit: Iowa Neuroscience Institute; Biology; Craniofacial Anomalies Research Center
Record Identifier: 9984070698902771

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