Chikungunya and O'nyong-nyong Viruses in Uganda: Implications for Diagnostics

Tamara L. Clements; Cynthia A. Rossi; Amanda K. Irish; Hannah Kibuuka; Leigh Anne Eller; Merlin L. Robb; Peter Kataaha; Nelson L. Michael; Lisa E. Hensley; Randal J. Schoepp

doi:10.1093/ofid/ofz001

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Chikungunya and O'nyong-nyong Viruses in Uganda: Implications for Diagnostics

Journal article

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Chikungunya and O'nyong-nyong Viruses in Uganda: Implications for Diagnostics

Tamara L. Clements, Cynthia A. Rossi, Amanda K. Irish, Hannah Kibuuka, Leigh Anne Eller, Merlin L. Robb, Peter Kataaha, Nelson L. Michael, Lisa E. Hensley and Randal J. Schoepp

Open forum infectious diseases, Vol.6(3), pp.ofz001-ofz001

03/01/2019

DOI: 10.1093/ofid/ofz001

PMCID: PMC6411207

PMID: 31660384

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Abstract

Background. A serosurvey of healthy blood donors provided evidence of hemorrhagic fever and arthropod-borne virus infections in Uganda. Methods. Antibody prevalence to arthropod-borne and hemorrhagic fever viruses in human sera was determined using enzyme-linked immunosorbent assay (ELISA) and plaque reduction neutralization test (PRNT). Results. The greatest antibody prevalence determined by ELISA was to chikungunya virus (CHIKV) followed in descending order by West Nile virus (WNV), Crimean-Congo hemorrhagic fever virus (CCHFV), Ebola virus (EBOV), dengue virus (DEN), yellow fever virus (YFV), Rift Valley fever virus (RVFV), Marburg virus (MARV), and Lassa virus (LASV). Further investigation of CHIKV-positive sera demonstrated that the majority of antibody responses may likely be the result of exposure to the closely related alphavirus o'nyong-nyong virus (ONNV). Conclusions. As the use of highly specific and sensitive polymerase chain reaction-based assays becomes the diagnostic standard without the corresponding use of the less sensitive but more broadly reactive immunological-based assays, emerging and re-emerging outbreaks will be initially missed, illustrating the need for an orthogonal system for the detection and identification of viruses causing disease.

Immunology

Infectious Diseases

Life Sciences & Biomedicine

Microbiology

Science & Technology

Details

Title: Subtitle: Chikungunya and O'nyong-nyong Viruses in Uganda: Implications for Diagnostics
Creators: Tamara L. Clements - United States Army Medical Research Institute of Infectious Diseases
Cynthia A. Rossi - United States Army Medical Research Institute of Infectious Diseases
Amanda K. Irish - University of Iowa
Hannah Kibuuka - Makerere University Walter Reed Project
Leigh Anne Eller - Makerere University Walter Reed Project
Merlin L. Robb - Henry M. Jackson Foundation
Peter Kataaha - Uganda Blood Transfusion Service
Nelson L. Michael - Walter Reed Army Institute of Research
Lisa E. Hensley - National Institute of Allergy and Infectious Diseases
Randal J. Schoepp - United States Army Medical Research Institute of Infectious Diseases
Resource Type: Journal article
Publication Details: Open forum infectious diseases, Vol.6(3), pp.ofz001-ofz001
DOI: 10.1093/ofid/ofz001
PMID: 31660384
PMCID: PMC6411207
NLM abbreviation: Open Forum Infect Dis
ISSN: 2328-8957
eISSN: 2328-8957
Publisher: Oxford Univ Press
Number of pages: 7
Grant note: P0023_16_RD; P0017_17_RD / Global Emerging Infections Surveillance Section of the Armed Forces Health Surveillance Branch (ProMIS plans) through US Army Medical Research Institute of Infectious Diseases US Military HIV Research Program through Walter Reed Army Institute of Research
Language: English
Date published: 03/01/2019
Academic Unit: Emergency Medicine
Record Identifier: 9984656609602771

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