Journal article
Chimpanzee GB virus C and GB virus A E2 envelope glycoproteins contain a peptide motif that inhibits human immunodeficiency virus type 1 replication in human CD4⁺ T-cells
Journal of general virology, Vol.94(Pt 4), pp.774-782
04/2013
DOI: 10.1099/vir.0.047126-0
PMCID: PMC3709681
PMID: 23288422
Abstract
GB virus type C (GBV-C) is a lymphotropic virus that can cause persistent infection in humans. GBV-C is not associated with any disease, but is associated with reduced mortality in human immunodeficiency virus type 1 (HIV-1)-infected individuals. Related viruses have been isolated from chimpanzees (GBV-Ccpz) and from New World primates (GB virus type A, GBV-A). These viruses are also capable of establishing persistent infection. We determined the nucleotide sequence encoding the envelope glycoprotein (E2) of two GBV-Ccpz isolates obtained from the sera of captive chimpanzees. The deduced GBV-Ccpz E2 protein differed from human GBV-C by 31 % at the amino acid level. Similar to human GBV-C E2, expression of GBV-Ccpz E2 in a tet-off human CD4(+) Jurkat T-cell line significantly inhibited the replication of diverse HIV-1 isolates. This anti-HIV-replication effect of GBV-Ccpz E2 protein was reversed by maintaining cells in doxycycline to reduce E2 expression. Previously, we found a 17 aa region within human GBV-C E2 that was sufficient to inhibit HIV-1. Although GBV-Ccpz E2 differed by 3 aa differences in this region, the chimpanzee GBV-C 17mer E2 peptide inhibited HIV-1 replication. Similarly, the GBV-A peptide that aligns with this GBV-C E2 region inhibited HIV-1 replication despite sharing only 5 aa with the human GBV-C E2 sequence. Thus, despite amino acid differences, the peptide region on both the GBV-Ccpz and the GBV-A E2 protein inhibit HIV-1 replication similar to human GBV-C. Consequently, GBV-Ccpz or GBV-A infection of non-human primates may provide an animal model to study GB virus-HIV interactions.
Details
- Title: Subtitle
- Chimpanzee GB virus C and GB virus A E2 envelope glycoproteins contain a peptide motif that inhibits human immunodeficiency virus type 1 replication in human CD4⁺ T-cells
- Creators
- James H McLinden - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USAJack T Stapleton - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USADonna Klinzman - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USAKrishna K Murthy - Department of Virology and Immunology, Texas Biomedical Research Institute, San Antonio, TX 78227, USAQing Chang - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USAThomas M Kaufman - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USANirjal Bhattarai - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USAJinhua Xiang - Department of Internal Medicine, Division of Infectious Diseases, Iowa City Veterans Affairs Medical Center and the University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Journal of general virology, Vol.94(Pt 4), pp.774-782
- DOI
- 10.1099/vir.0.047126-0
- PMID
- 23288422
- PMCID
- PMC3709681
- NLM abbreviation
- J Gen Virol
- ISSN
- 0022-1317
- eISSN
- 1465-2099
- Grant note
- R01 AI-58740 / NIAID NIH HHS R01 AI058740 / NIAID NIH HHS P51 OD011133 / NIH HHS
- Language
- English
- Date published
- 04/2013
- Academic Unit
- Microbiology and Immunology; Infectious Diseases; Internal Medicine
- Record Identifier
- 9984094558702771
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