Journal article
Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
Nanotechnology, Vol.24(39), pp.395101-395101
10/04/2013
DOI: 10.1088/0957-4484/24/39/395101
PMCID: PMC3816956
PMID: 24008224
Abstract
Despite their potential for a variety of applications, copper nanoparticles induce very strong inflammatory responses and cellular toxicity following aerosolized delivery. Coating metallic nanoparticles with polysaccharides, such as biocompatible and antimicrobial chitosan, has the potential to reduce this toxicity. In this study, copper nanoparticles were coated with chitosan using a newly developed and facile method. The presence of coating was confirmed using x-ray photoelectron spectroscopy, rhodamine tagging of chitosan followed by confocal fluorescence imaging of coated particles and observed increases in particle size and zeta potential. Further physical and chemical characteristics were evaluated using dissolution and x-ray diffraction studies. The chitosan coating was shown to significantly reduce the toxicity of copper nanoparticles after 24 and 52 h and the generation of reactive oxygen species as assayed by DHE oxidation after 24 h in vitro. Conversely, inflammatory response, measured using the number of white blood cells, total protein, and cytokines/chemokines in the bronchoalveolar fluid of mice exposed to chitosan coated versus uncoated copper nanoparticles, was shown to increase, as was the concentration of copper ions. These results suggest that coating metal nanoparticles with mucoadhesive polysaccharides (e.g. chitosan) could increase their potential for use in controlled release of copper ions to cells, but will result in a higher inflammatory response if administered via the lung.
Details
- Title: Subtitle
- Chitosan coating of copper nanoparticles reduces in vitro toxicity and increases inflammation in the lung
- Creators
- Kristan L S Worthington - Department of Chemical and Biochemical Engineering, College of Engineering, University of Iowa, Iowa City, IA 52242, USA. Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USAAndrea Adamcakova-DoddAmaraporn WongrakpanichImali A MudunkotuwaKranti A MapuskarVijaya B JoshiC Allan Guymon - University of Iowa, Chemical and Biochemical EngineeringDouglas R SpitzVicki H GrassianPeter S ThorneAliasger K Salem
- Resource Type
- Journal article
- Publication Details
- Nanotechnology, Vol.24(39), pp.395101-395101
- DOI
- 10.1088/0957-4484/24/39/395101
- PMID
- 24008224
- PMCID
- PMC3816956
- NLM abbreviation
- Nanotechnology
- ISSN
- 0957-4484
- eISSN
- 1361-6528
- Publisher
- IOP Publishing; England
- Grant note
- P30 ES005605 / NIEHS NIH HHS 1R21CA13345-01 / NCI NIH HHS P30ES005605 / NIEHS NIH HHS 1R21CA128414-01A2/UI / NCI NIH HHS R21 CA128414 / NCI NIH HHS P30CA086862 / NCI NIH HHS P30 CA086862 / NCI NIH HHS R21 CA133456 / NCI NIH HHS
- Language
- English
- Date published
- 10/04/2013
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Pathology; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Radiation Oncology; Craniofacial Anomalies Research Center; Chemistry; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983985830402771
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