Chitosan gallate as a novel potential polysaccharide antioxidant: an EPR study

Wanvimol Pasanphan; Garry R Buettner; Suwabun Chirachanchai

doi:10.1016/j.carres.2009.09.038

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Chitosan gallate as a novel potential polysaccharide antioxidant: an EPR study

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Chitosan gallate as a novel potential polysaccharide antioxidant: an EPR study

Wanvimol Pasanphan, Garry R Buettner and Suwabun Chirachanchai

Carbohydrate research, Vol.345(1), pp.132-140

2010

DOI: 10.1016/j.carres.2009.09.038

PMCID: PMC3695485

PMID: 19889400

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Abstract

A novel biopolymer-based antioxidant, chitosan conjugated with gallic acid (chitosan galloylate, chitosan–GA), is proposed. Electron paramagnetic resonance (EPR) demonstrates a wide range of antioxidant activity for chitosan–GA as evidenced from its reactions with oxidizing free radicals, that is, 1,1-diphenyl-2-picryl-hydrazyl (DPPH), horseradish peroxidase (HRP)–H 2O 2, carbon-centered alkyl radicals, and hydroxyl radicals. The EPR spectrum of the radical formed on chitosan–GA was attributed to the semiquinone radical of the gallate moiety. The stoichiometry and effective concentration (EC 50) of the DPPH free radical with chitosan–GA show that the radical scavenging capacity is maintained even after thermal treatment at 100 °C for an hour. Although the degree of substitution of GA on chitosan was about 15%, its antioxidant capacity, that is, the reaction with carbon-centered and hydroxyl radicals, is comparable to that of GA.

Gallic acid (GA)

Chitosan

Antioxidant

Polysaccharide

Electron paramagnetic resonance (EPR)

Details

Title: Subtitle: Chitosan gallate as a novel potential polysaccharide antioxidant: an EPR study
Creators: Wanvimol Pasanphan - The Department of Applied Radiation and Isotopes, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
Garry R Buettner - Free Radical and Radiation Biology, ESR Facility, The University of Iowa, Iowa City, IA 52242-1181, USA
Suwabun Chirachanchai - The Petroleum and Petrochemical College, Chulalongkorn University, Bangkok 10330, Thailand
Resource Type: Journal article
Publication Details: Carbohydrate research, Vol.345(1), pp.132-140
DOI: 10.1016/j.carres.2009.09.038
PMID: 19889400
PMCID: PMC3695485
NLM abbreviation: Carbohydr Res
ISSN: 0008-6215
eISSN: 1873-426X
Publisher: Elsevier Ltd
Language: English
Date published: 2010
Academic Unit: Radiation Oncology
Record Identifier: 9984047618002771

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