Chitosan malate inhibits growth and exotoxin production of toxic shock syndrome-inducing Staphylococcus aureus strains and group A streptococci

Patrick M Schlievert

doi:10.1128/AAC.01295-06

Back

Chitosan malate inhibits growth and exotoxin production of toxic shock syndrome-inducing Staphylococcus aureus strains and group A streptococci

Journal article

Open access

Peer reviewed

Chitosan malate inhibits growth and exotoxin production of toxic shock syndrome-inducing Staphylococcus aureus strains and group A streptococci

Patrick M Schlievert

Antimicrobial agents and chemotherapy, Vol.51(9), pp.3056-3062

09/2007

DOI: 10.1128/AAC.01295-06

PMCID: PMC2043168

PMID: 17576832

Files and links (1)

url

https://doi.org/10.1128/AAC.01295-06View

Published (Version of record) Open Access

Abstract

Previously, it has been shown that the polysaccharide chitosan inhibits the growth of gram-positive bacteria. In this study, chitosan malate was evaluated in broth and thin-film cultures for its effect on the growth and exotoxin production of toxic shock syndrome (TSS)-inducing Staphylococcus aureus (five strains, three producing TSS toxin 1 and one each producing enterotoxin B or C) and group A streptococci (three strains producing streptococcal pyrogenic exotoxin A). Also, the compound was evaluated in a rabbit subcutaneous Wiffle ball model for its ability to prevent S. aureus and group A streptococcal induction of TSS. Finally, chitosan malate was evaluated for its ability to prevent TSS and necrotizing fasciitis in rabbits after subcutaneous inoculation with microbes. Chitosan malate inhibited both bacterial growth and, at sub-growth-inhibitory concentrations, the production of exotoxins, in both broth and thin-film cultures. Rabbits treated with chitosan malate in implanted Wiffle balls were protected from prior challenge with TSS-inducing S. aureus compared to animals not receiving chitosan malate (P < 0.001) and group A streptococci (P < 0.005). Chitosan malate protected rabbits from the development of streptococcal TSS with necrotizing fasciitis (P < 0.01). The data suggest that use of this growth- and toxin-inhibitory compound may be able to reduce the severity of S. aureus and group A streptococcal mucous membrane and trauma-associated skin infections.

Streptococcus pyogenes - growth & development

Male

Colony Count, Microbial

Anti-Bacterial Agents

Chitosan - therapeutic use

Shock, Septic - drug therapy

Chitosan - pharmacology

Female

Hemolysin Proteins - biosynthesis

Chitosan - analogs & derivatives

Malates - toxicity

Staphylococcus aureus - metabolism

Rabbits

Streptococcus pyogenes - drug effects

Enzyme-Linked Immunosorbent Assay

Malates - pharmacology

Streptococcus pyogenes - metabolism

Chitosan - toxicity

Shock, Septic - microbiology

Exotoxins - biosynthesis

Animals

Fasciitis, Necrotizing - prevention & control

Fasciitis, Necrotizing - microbiology

Bacterial Proteins - biosynthesis

Staphylococcus aureus - drug effects

Staphylococcus aureus - growth & development

Malates - therapeutic use

Details

Title: Subtitle: Chitosan malate inhibits growth and exotoxin production of toxic shock syndrome-inducing Staphylococcus aureus strains and group A streptococci
Creators: Patrick M Schlievert - Department of Microbiology, University of Minnesota Medical School, MMC 196, 420 Delaware St. SE, Minneapolis, MN 55455, USA. schli001@umn.edu
Resource Type: Journal article
Publication Details: Antimicrobial agents and chemotherapy, Vol.51(9), pp.3056-3062
DOI: 10.1128/AAC.01295-06
PMID: 17576832
PMCID: PMC2043168
NLM abbreviation: Antimicrob Agents Chemother
ISSN: 0066-4804
eISSN: 1098-6596
Publisher: United States
Grant note: HL-36611 / NHLBI NIH HHS R01 HL036611 / NHLBI NIH HHS
Language: English
Date published: 09/2007
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001206702771

Metrics

22 Record Views

12 Times Cited - Web of Science