Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts

Ali Fakhry; Galen B Schneider; Rebecca Zaharias; Sevda Senel

doi:10.1016/j.biomaterials.2003.08.068

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Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts

Journal article

Peer reviewed

Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts

Ali Fakhry, Galen B Schneider, Rebecca Zaharias and Sevda Senel

Biomaterials, Vol.25(11), pp.2075-2079

05/2004

DOI: 10.1016/j.biomaterials.2003.08.068

PMID: 14741622

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Abstract

The aim of this study was to determine chitosan's effect on osteoblast and fibroblast cell attachment. Mouse MC3T3-E1 osteoblasts and 3T3 fibroblasts were grown in the presence of serum on two commercially available chitosans, Chitosan-H (CH) and Protasan CL212 (PR). Cell attachment and immunofluorescent analysis at various time points were done to analyze initial phenotypic profiles. At 1h, significantly (P<0.05) fewer fibroblasts attached to CH or PR than serum-coated substrates. Osteoblast attachment to the same biopolymers at 1h was significantly greater than those seen with fibroblasts. At 24 h, levels of cell attachment for fibroblasts to both CH and PR significantly increased and were similar to levels seen in osteoblast cultures at both 1 and 24 h. Morphologically, immunofluorescent analysis showed that osteoblasts plated on the biopolymers were attached and beginning to spread at 1h, whereas fibroblasts appeared more rounded. At 24 h, fibroblasts plated on CH or PR revealed a heterogeneous population of round and semi-spread cells. In comparison, osteoblasts displayed phenotypes that were well spread with a developed cytoskeleton. These results suggest that CH and PR support the initial attachment and spreading of osteoblasts preferentially over fibroblasts, and that manipulation of the biopolymer can alter the level of attachment and spreading.

Cell Line

Animals

Cell Adhesion - physiology

Fibroblasts - physiology

Osteoblasts - physiology

Coated Materials, Biocompatible - chemistry

Materials Testing

Fibroblasts - cytology

Mice

Osteoblasts - cytology

3T3 Cells

Cell Movement - physiology

Details

Title: Subtitle: Chitosan supports the initial attachment and spreading of osteoblasts preferentially over fibroblasts
Creators: Ali Fakhry - Department of Periodontics, College of Dentistry, University of Iowa, DSB 450, Iowa City, IA 52242, USA. ali-fakhry@uiowa.edu
Galen B Schneider
Rebecca Zaharias
Sevda Senel
Resource Type: Journal article
Publication Details: Biomaterials, Vol.25(11), pp.2075-2079
Publisher: Netherlands
DOI: 10.1016/j.biomaterials.2003.08.068
PMID: 14741622
ISSN: 0142-9612
eISSN: 1878-5905
Grant note: P30-DE10126-10 / NIDCR NIH HHS
Language: English
Date published: 05/2004
Academic Unit: Dentistry Administration; Prosthodontics
Record Identifier: 9984066080602771

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