Journal article
Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection
Cell reports (Cambridge), Vol.26(12), pp.3380-3390.e5
03/19/2019
DOI: 10.1016/j.celrep.2019.02.079
PMID: 30893609
Abstract
Chlamydial infection requires the formation of a membrane-bound vacuole, termed the inclusion, that undergoes extensive interactions with select host organelles. The importance of the Inc protein CT229 in the formation and maintenance of the chlamydial inclusion was recently highlighted by studies demonstrating that its absence during infection results in reduced bacterial replication, premature inclusion lysis, and host cell death. Previous reports have indicated that CT229 binds Rab GTPases; however, the physiological implications of this interaction are unknown. Here, we show that CT229 regulates host multivesicular trafficking by recruiting multiple Rab GTPases and their cognate effectors to the inclusion. We demonstrate that CT229 specifically modulates clathrin-coated vesicle trafficking and regulates the trafficking of transferrin and the mannose-6-phosphate receptor, both of which are crucial for proper chlamydial development. This study highlights CT229 as a master regulator of multiple host vesicular trafficking pathways essential for chlamydial infection.
Details
- Title: Subtitle
- Chlamydia trachomatis CT229 Subverts Rab GTPase-Dependent CCV Trafficking Pathways to Promote Chlamydial Infection
- Creators
- Robert Faris - Roy J. and Lucille A. Carver College of MedicineMarlena Merling - Roy J. and Lucille A. Carver College of MedicineShelby E. Andersen - Roy J. and Lucille A. Carver College of MedicineCheryl A. Dooley - Rocky Mountain LaboratoriesTed Hackstadt - Rocky Mountain LaboratoriesMary M. Weber - Roy J. and Lucille A. Carver College of Medicine
- Resource Type
- Journal article
- Publication Details
- Cell reports (Cambridge), Vol.26(12), pp.3380-3390.e5
- Publisher
- Elsevier
- DOI
- 10.1016/j.celrep.2019.02.079
- PMID
- 30893609
- ISSN
- 2211-1247
- eISSN
- 2211-1247
- Number of pages
- 16
- Grant note
- ZIAAI000567 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) University of Iowa
- Language
- English
- Date published
- 03/19/2019
- Academic Unit
- Molecular Physiology and Biophysics; Microbiology and Immunology
- Record Identifier
- 9984297426002771
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