Journal article
Choice of 3' cleavage/polyadenylation site in β-tropomyosin RNA processing is differentiation-dependent in mouse BC3H1 muscle cells
The Journal of biological chemistry, Vol.267(4), pp.2728-2736
1992
DOI: 10.1016/S0021-9258(18)45940-6
PMID: 1733968
Abstract
The rodent beta-tropomyosin (TM) gene produces either a 1.2-kilobase (kb) skeletal muscle beta-TM mRNA or a 1.1-kb fibroblast/smooth muscle TM-1 mRNA through tissue-specific alternative exon splicing and 3' cleavage/polyadenylation at two alternative poly(A) sites. beta-TM mRNA contains exon 6b, 9a, and the poly(A) site immediately following exon 9a, whereas TM-1 mRNA contains exon 6a, 9b, and the poly(A) site following exon 9b. We isolated a novel 2.1-kb beta-TM cDNA clone, pUTM, from a cDNA library of 2-day differentiated mouse BC3H1 muscle-like cells. This cDNA contains the entire sequence of mature beta-TM mRNA with a normal but unused poly(A) site associated with exon 9a. Instead, 3' cleavage/polyadenylation of this cDNA occurred at the exon 9b-associated distal poly(A) site, resulting in the retention of a 1-kb intron and the TM-1 exon 9b. We identified a 2.3-kb functional mRNA, UTM RNA, corresponding to pUTM. UTM RNA appeared early during BC3H1 cell differentiation and gradually decreased as the beta-TM mRNA increased. UTM RNA was also detected in mouse C2C12 muscle cells and in skeletal muscle tissue isolated from mouse leg. Thus, in the processing of beta-TM gene transcripts, selection of alternative terminal exons and alternative poly(A) sites are not necessarily linked as they appear to be in other gene systems.
Details
- Title: Subtitle
- Choice of 3' cleavage/polyadenylation site in β-tropomyosin RNA processing is differentiation-dependent in mouse BC3H1 muscle cells
- Creators
- YUNG-CHIH WANG - Univ. Iowa coll. medicine, cardiovascular res. cent., dep. biochemistry, Iowa City IA 52242, United StatesP. A RUBENSTEIN - Univ. Iowa coll. medicine, cardiovascular res. cent., dep. biochemistry, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.267(4), pp.2728-2736
- DOI
- 10.1016/S0021-9258(18)45940-6
- PMID
- 1733968
- NLM abbreviation
- J Biol Chem
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Publisher
- American Society for Biochemistry and Molecular Biology; Bethesda, MD
- Language
- English
- Date published
- 1992
- Academic Unit
- Stead Family Department of Pediatrics; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984025272802771
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