Journal article
Cholinergic receptor induction and JNK activation in acute pancreatitis
The American journal of surgery, Vol.186(5), pp.569-574
2003
DOI: 10.1016/j.amjsurg.2003.07.016
PMID: 14599627
Abstract
Cholecystokinin-A (CCK-A) and cholinergic receptor pathways, capable of activating stress kinases p38 mitogen-activated protein kinase (p38
MAPK) and cJUN N-terminal kinase (JNK), are implicated in the pathogenesis of ligation-induced acute pancreatitis in rats. As ligation-induced acute pancreatitis in rats is associated with CCK-A receptor induction and p38
MAPK activation, and as receptor induction could amplify acinar hyperstimulation and exacerbate cell stress, we tested the hypothesis that the cholinergic M3 receptor is induced and JNK is activated in this model.
Cholinergic M3 receptor expression and JNK activation was compared in rats 1, 3, or 24 hours after sham operation or duct ligation.
Immunoblot analysis of pancreatic homogenates showed a time-dependent increase in cholinergic M3 receptor protein, total JNK, and phospho-JNK after duct ligation.
There is a rapid and progressive cholinergic M3 receptor induction and JNK activation in ligation-induced acute pancreatitis in rats. These findings may have significance in the mechanism of disease pathogenesis.
Details
- Title: Subtitle
- Cholinergic receptor induction and JNK activation in acute pancreatitis
- Creators
- Isaac Samuel - Department of Surgery, Veterans Affairs Medical Center, University of Iowa Roy J. and Lucille A. Carver College of Medicine, 200 Hawkins Dr., 4625 JCP, Iowa City, IA 52242, USASmita Zaheer - Department of Surgery, Veterans Affairs Medical Center, University of Iowa Roy J. and Lucille A. Carver College of Medicine, 200 Hawkins Dr., 4625 JCP, Iowa City, IA 52242, USARory A Fisher - Department of Pharmacology, University of Iowa Roy J. and Lucille A. Carver College of Medicine, Iowa City, IA, USAAsgar Zaheer - Department of Neurology, University of Iowa Roy J. and Lucille A. Carver College of Medicine and the Veterans Affairs Medical Center, Iowa City, IA, USA
- Resource Type
- Journal article
- Publication Details
- The American journal of surgery, Vol.186(5), pp.569-574
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.amjsurg.2003.07.016
- PMID
- 14599627
- ISSN
- 0002-9610
- eISSN
- 1879-1883
- Language
- English
- Date published
- 2003
- Academic Unit
- Neurology; Iowa Neuroscience Institute; Surgery; Neuroscience and Pharmacology; Internal Medicine
- Record Identifier
- 9984040320402771
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