Journal article
Chondrogenic progenitor cells respond to cartilage injury
Arthritis and rheumatism, Vol.64(11), pp.3626-3637
11/2012
DOI: 10.1002/art.34613
PMCID: PMC4950521
PMID: 22777600
Abstract
Hypocellularity resulting from chondrocyte death in the aftermath of mechanical injury is thought to contribute to posttraumatic osteoarthritis. However, we observed that nonviable areas in cartilage injured by blunt impact were repopulated within 7-14 days by cells that appeared to migrate from the surrounding matrix. The aim of this study was to assess our hypothesis that the migrating cell population included chondrogenic progenitor cells that were drawn to injured cartilage by alarmins.
Osteochondral explants obtained from mature cattle were injured by blunt impact or scratching, resulting in localized chondrocyte death. Injured sites were serially imaged by confocal microscopy, and migrating cells were evaluated for chondrogenic progenitor characteristics. Chemotaxis assays were used to measure the responses to chemokines, injury-conditioned medium, dead cell debris, and high mobility group box chromosomal protein 1 (HMGB-1).
Migrating cells were highly clonogenic and multipotent and expressed markers associated with chondrogenic progenitor cells. Compared with chondrocytes, these cells overexpressed genes involved in proliferation and migration and underexpressed cartilage matrix genes. They were more active than chondrocytes in chemotaxis assays and responded to cell lysates, conditioned medium, and HMGB-1. Glycyrrhizin, a chelator of HMGB-1 and a blocking antibody to receptor for advanced glycation end products (RAGE), inhibited responses to cell debris and conditioned medium and reduced the numbers of migrating cells on injured explants.
Injuries that caused chondrocyte death stimulated the emergence and homing of chondrogenic progenitor cells, in part via HMGB-1 release and RAGE-mediated chemotaxis. Their repopulation of the matrix could promote the repair of chondral damage that might otherwise contribute to progressive cartilage loss.
Details
- Title: Subtitle
- Chondrogenic progenitor cells respond to cartilage injury
- Creators
- Dongrim Seol - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityDaniel J McCabe - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityHyeonghun Choe - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityHongjun Zheng - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityYin Yu - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityKeewoong Jang - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityMorgan W Walter - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityAbigail D Lehman - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityLei Ding - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa CityJoseph A Buckwalter - Joseph A. Buckwalter, MD: University of Iowa and VA Medical Center, Iowa City, IowaJames A Martin - Dongrim Seol, PhD, Daniel J. McCabe, BS, Hyeonghun Choe, ME, Hongjun Zheng, PhD, Yin Yu, BM, Keewoong Jang, MS, Morgan W. Walter, BS, Abigail D. Lehman, BS, Lei Ding, PhD, James A. Martin, PhD: University of Iowa, Iowa City
- Resource Type
- Journal article
- Publication Details
- Arthritis and rheumatism, Vol.64(11), pp.3626-3637
- DOI
- 10.1002/art.34613
- PMID
- 22777600
- PMCID
- PMC4950521
- NLM abbreviation
- Arthritis Rheum
- ISSN
- 0004-3591
- eISSN
- 1529-0131
- Publisher
- Wiley; United States
- Grant note
- P50 AR055533 / NIAMS NIH HHS
- Language
- English
- Date published
- 11/2012
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Orthodontics; Stead Family Department of Pediatrics; Emergency Medicine; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation; Injury Prevention Research Center
- Record Identifier
- 9984040269902771
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