Journal article
Chondroitin sulfate B exerts its inhibitory effect on secondary lymphoid tissue chemokine (SLC) by binding to the C-terminus of SLC
Biochimica et biophysica acta. General subjects, Vol.1571(3), pp.219-224
2002
DOI: 10.1016/S0304-4165(02)00232-5
PMID: 12090936
Abstract
We previously reported that certain glycosaminoglycans (GAGs) bind secondary lymphoid tissue chemokine (SLC, CCL21) and that the SLC-binding GAGs, including chondroitin sulfate B (CS B), negatively modulate the function of SLC, although the mechanism remains unknown [J. Biol. Chem. 276 (2001) 5228]. To gain insight into the mechanism of inhibition, we used a C-terminally truncated SLC (SLC-T) that lacked clusters of basic amino acid residues that have been implicated in GAG binding. While SLC-T failed to bind any GAGs, it induced prominent intracellular Ca
2+ mobilization in CC chemokine receptor (CCR) 7-expressing cells, as did wild-type SLC. However, the SLC-T-induced Ca
2+ influx was not inhibited by CS B, unlike the SLC-induced Ca
2+ influx. These results demonstrate the requirement of the C-terminus of SLC for the inhibition of chemokine responses by CS B; that is, CS B exerts its inhibitory effect by binding to the C-terminus of SLC, thus defining the mode of action of CS B on certain chemokines.
Details
- Title: Subtitle
- Chondroitin sulfate B exerts its inhibitory effect on secondary lymphoid tissue chemokine (SLC) by binding to the C-terminus of SLC
- Creators
- Jun Hirose - Osaka UniversityHiroto Kawashima - Osaka UniversityMelissa Swope Willis - Harvard Medical SchoolTimothy A Springer - Harvard UniversityHitoshi Hasegawa - Ehime UniversityOsamu Yoshie - Kindai UniversityMasayuki Miyasaka - Osaka University
- Resource Type
- Journal article
- Publication Details
- Biochimica et biophysica acta. General subjects, Vol.1571(3), pp.219-224
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0304-4165(02)00232-5
- PMID
- 12090936
- ISSN
- 0304-4165
- eISSN
- 1872-8006
- Grant note
- DOI: 10.13039/501100001700, name: Ministry of Education, Culture, Sports, Science and Technology; DOI: 10.13039/501100002241, name: Japan Science and Technology Agency
- Language
- English
- Date published
- 2002
- Academic Unit
- Dermatology
- Record Identifier
- 9984296307502771
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