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Chromium-induced cross-linking of nuclear proteins and DNA
Journal article   Open access   Peer reviewed

Chromium-induced cross-linking of nuclear proteins and DNA

Andrzej Wedrychowski, W Steven Ward, Warren N Schmidt and Lubomir S Hnilica
The Journal of biological chemistry, Vol.260(11), pp.7150-7155
1985
DOI: 10.1016/S0021-9258(18)88901-3
PMID: 3997861
url
https://doi.org/10.1016/S0021-9258(18)88901-3View
Published (Version of record) Open Access

Abstract

The in vivo cross-linking of proteins to DNA in intact Novikoff ascites hepatoma cells exposed to the chromium salt K2CrO4 was studied. DNA-protein complexes were assayed by high speed centrifugation of cells solubilized in buffered 4% sodium dodecyl sulfate and by electrophoretic identification of proteins associated with DNA-containing pellets. Further evidence of DNA-protein complexes, not dissociable in this buffer, was obtained by CsCl gradient centrifugation. Time dependence experiments showed that detectable cross-linking occurred after cells were exposed to chromium salt for at least 4 h, and the amount of DNA-protein complexes increased with longer incubation times. Complex formation occurred only with chromium salt concentrations of 200 microM or greater, and maximal cross-linking was effected at 5 mM. Immunotransfer methodology employing antibodies to nuclear matrix fraction and lamins was used to identify some of the polypeptides comprising the cross-linked complexes. These studies indicated specificity of chromium-induced complex formation within the nuclear protein fractions assayed. Our results document the ability of chromate to produce specific DNA-protein cross-links in living cells.
 Toxicology Biological and medical sciences Medical sciences Chemical and industrial products toxicology. Toxic occupational diseases Metals and various inorganic compounds

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