Chromophore supply rate-limits mammalian photoreceptor dark adaptation

Jin-shan Wang; Soile Nymark; Rikard Frederiksen; Maureen E Estevez; Susan Q Shen; Joseph C Corbo; M Carter Cornwall; Vladimir J Kefalov

doi:10.1523/jneurosci.1245-14.2014

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Chromophore supply rate-limits mammalian photoreceptor dark adaptation

Journal article

Open access

Peer reviewed

Chromophore supply rate-limits mammalian photoreceptor dark adaptation

Jin-shan Wang, Soile Nymark, Rikard Frederiksen, Maureen E Estevez, Susan Q Shen, Joseph C Corbo, M Carter Cornwall and Vladimir J Kefalov

The Journal of neuroscience, Vol.34(34), pp.11212-11221

08/20/2014

DOI: 10.1523/jneurosci.1245-14.2014

PMCID: PMC4138333

PMID: 25143602

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Abstract

Efficient regeneration of visual pigment following its destruction by light is critical for the function of mammalian photoreceptors. Here, we show that misexpression of a subset of cone genes in the rd7 mouse hybrid rods enables them to access the normally cone-specific retina visual cycle. The rapid supply of chromophore by the retina visual cycle dramatically accelerated the mouse rod dark adaptation. At the same time, the competition between rods and cones for retina-derived chromophore slowed cone dark adaptation, indicating that the cone specificity of the retina visual cycle is key for rapid cone dark adaptation. Our findings demonstrate that mammalian photoreceptor dark adaptation is dominated by the supply of chromophore. Misexpression of cone genes in rods may represent a novel approach to treating visual disorders associated with mutations of visual cycle proteins or with reduced retinal pigment epithelium function due to aging.

Action Potentials - physiology

Animals

Dark Adaptation - physiology

Female

GTP-Binding Protein alpha Subunits - genetics

Male

Mice

Mice, Inbred C57BL

Mice, Transgenic

Neuroglia - physiology

Orphan Nuclear Receptors - genetics

Photic Stimulation

Retina - cytology

Retina - radiation effects

Retinal Cone Photoreceptor Cells - physiology

Retinal Degeneration - genetics

Retinal Degeneration - pathology

Retinal Rod Photoreceptor Cells - physiology

Retinal Rod Photoreceptor Cells - radiation effects

Rhodopsin - genetics

Rhodopsin - metabolism

Time Factors

Transducin - genetics

Vitamin A - pharmacology

Vitamins - pharmacology

Details

Title: Subtitle: Chromophore supply rate-limits mammalian photoreceptor dark adaptation
Creators: Jin-shan Wang - Departments of Ophthalmology and Visual Sciences and
Soile Nymark - Boston University
Rikard Frederiksen - Boston University
Maureen E Estevez - Boston University
Susan Q Shen - Washington University in St. Louis
Joseph C Corbo - Washington University in St. Louis
M Carter Cornwall - Boston University
Vladimir J Kefalov - Departments of Ophthalmology and Visual Sciences and kefalov@wustl.edu
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.34(34), pp.11212-11221
DOI: 10.1523/jneurosci.1245-14.2014
PMID: 25143602
PMCID: PMC4138333
NLM abbreviation: J Neurosci
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: R01 HG006790 / NHGRI NIH HHS R01 EY001157 / NEI NIH HHS R21 HG006346 / NHGRI NIH HHS HG006790 / NHGRI NIH HHS EY01157 / NEI NIH HHS R01 EY018826 / NEI NIH HHS R24 EY021126 / NEI NIH HHS EY021126 / NEI NIH HHS EY18826 / NEI NIH HHS P30 EY002687 / NEI NIH HHS R01 EY019312 / NEI NIH HHS HG006346 / NHGRI NIH HHS EY019312 / NEI NIH HHS EY002687 / NEI NIH HHS
Language: English
Date published: 08/20/2014
Academic Unit: Psychiatry; Iowa Neuroscience Institute
Record Identifier: 9984618637402771

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