Journal article
Chromosomal Aneusomies Detected by Fluorescent in Situ Hybridization Analysis in Clinically Localized ProstateCarcinoma
The Journal of urology, Vol.152(4), pp.1157-1162
10/1994
DOI: 10.1016/S0022-5347(17)32527-2
Abstract
Fluorescent in situ hybridization using 12 chromosome enumeration probes (for chromosomes 4, 6, 7, 8, 9, 10, 11, 12, 17, 18, X and Y) was used to evaluate fresh tumor touch preparations from 40 randomly selected radical prostatectomy specimens. Of the tumors 16 (40%) contained chromosomal aneusomies. Chromosome 8 was aneusomic in 9 tumors (23%). Gain of chromosome 7 was observed in 8 tumors (20%). Chromosome 17 was aneusomic in 4 cases, and chromosomes 10, 11, 12, 18 and Y were each aneusomic twice. Loss of chromosome 9 was observed in 1 tumor. Chromosomes 4, 6, and X were never aneusomic. The percentage of monosomy 17 nuclei was 2 to 4 times the amount noted with the other autosomes for tumor and benign tissue. Computer analysis demonstrated that these signals contained twice the signal density and were significantly different (p <0.0001) than the single diploid chromosome 17 signals. This result is consistent with homologous pairing of chromosome 17 in benign and neoplastic prostate tissue. Anomalies of chromosomes 8 and/or 7 were present in 14 of the 16 cases (88%) aneusomic by fluorescent in situ hybridization. High grade tumors were more likely to be aneuploid on fluorescent in situ hybridization (p <0.001). Tumors with chromosome 8 aneusomies were of higher stage (p <0.05). Fluorescent in situ hybridization is more sensitive than flow cytometry for the detection of aneusomy/aneuploidy. The prognostic relevance of these findings will require further investigation.
Anomalies of chromosomes 8 and/or 7 were present in 14 of the 16 cases (88%) aneusomic by fluorescent in situ hybridization. High grade tumors were more likely to be aneuploid on fluorescent in situ hybridization (p <0.001). Tumors with chromosome 8 aneusomies were of higher stage (p <0.05). Fluorescent in situ hybridization is more sensitive than flow cytometry for the detection of aneusomy/aneuploidy. The prognostic relevance of these findings will require further investigation.
Details
- Title: Subtitle
- Chromosomal Aneusomies Detected by Fluorescent in Situ Hybridization Analysis in Clinically Localized ProstateCarcinoma
- Creators
- James A. Brown - Mayo Clinic in FloridaAntonio Alcaraz - Mayo ClinicSatoru Takahashi - Mayo ClinicDiane L. Persons - Mayo ClinicMichael M. Lieber - Mayo ClinicRobert B. Jenkins - Mayo Clinic
- Resource Type
- Journal article
- Publication Details
- The Journal of urology, Vol.152(4), pp.1157-1162
- DOI
- 10.1016/S0022-5347(17)32527-2
- ISSN
- 0022-5347
- eISSN
- 1527-3792
- Language
- English
- Date published
- 10/1994
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Urology
- Record Identifier
- 9984320069502771
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