Journal article
Chromosomal Assignment of 2900 Tri- and Tetranucleotide Repeat Markers Using NIGMS Somatic Cell Hybrid Panel 2
Genomics (San Diego, Calif.), Vol.32(1), pp.15-20
02/15/1996
DOI: 10.1006/geno.1996.0071
PMID: 8786107
Abstract
Two thousand nine hundred and thirty-one tri- and tetranucleotide short tandem repeat polymorphisms (STRPs) developed by the Cooperative Human Linkage Center were assigned to chromosomes using the NIGMS somatic cell hybrid mapping panel 2 and an efficient pooling strategy. Approximately 82% of all STRPs tested were assigned by this method, with 96.7% accuracy. Many of the single chromosome cell lines contained portions of additional chromosomes, confirming previous reports. The cell lines for chromosomes 6, 14, and 20 contained extensive portions of other chromosomes. Five previously unreported chromosomal contaminants were identified and are reported. A new pooling strategy was designed to minimize ambiguous assignments.
Details
- Title: Subtitle
- Chromosomal Assignment of 2900 Tri- and Tetranucleotide Repeat Markers Using NIGMS Somatic Cell Hybrid Panel 2
- Creators
- Sara L.F Sunden - Department of Pediatrics, University of Iowa, Iowa City, Iowa, 52242Tom Businga - Department of Pediatrics, University of Iowa, Iowa City, Iowa, 52242John Beck - Department of Pediatrics, University of Iowa, Iowa City, Iowa, 52242Ann McClain - Department of Pediatrics, University of Iowa, Iowa City, Iowa, 52242Julie M Gastier - Department of Genetics, Harvard Medical School, Harvard Medical Institute, Boston, Massachusetts, 02115Jacqueline C Pulido - Howard Hughes Medical Institute, Harvard Medical Institute, Harvard Medical School, Boston, Massachusetts, 02115Chandri Naidu Yandava - Department of Genetics, Harvard Medical School, Harvard Medical Institute, Boston, Massachusetts, 02115Thomas Brody - Department of Genetics, Harvard Medical School, Harvard Medical Institute, Boston, Massachusetts, 02115Jelveh Ghazizadeh - Howard Hughes Medical Institute, Harvard Medical Institute, Harvard Medical School, Boston, Massachusetts, 02115James L Weber - Marshfield Medical Research Foundation, Marshfield, Wisconsin, 54449Geoffrey M Duyk - Department of Genetics, Harvard Medical School, Harvard Medical Institute, Boston, Massachusetts, 02115Jeffrey C Murray - Department of Pediatrics, University of Iowa, Iowa City, Iowa, 52242Kenneth H Buetow - Fox Chase Cancer Center, Philadelphia, Pennsylvania, 19111V.C Sheffield - Department of Pediatrics, University of Iowa, Iowa City, Iowa, 52242
- Resource Type
- Journal article
- Publication Details
- Genomics (San Diego, Calif.), Vol.32(1), pp.15-20
- DOI
- 10.1006/geno.1996.0071
- PMID
- 8786107
- NLM abbreviation
- Genomics
- ISSN
- 0888-7543
- eISSN
- 1089-8646
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 02/15/1996
- Academic Unit
- Anatomy and Cell Biology; Stead Family Department of Pediatrics; Epidemiology; Pathology; Iowa Neuroscience Institute; Medical Genetics and Genomics; Pediatric Dentistry; Craniofacial Anomalies Research Center; Dental Research; Ophthalmology and Visual Sciences
- Record Identifier
- 9984025480702771
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