Journal article
Chromosome 1 analysis in chromophobe renal cell carcinomas with tissue microarray (TMA)-facilitated fluorescence in situ hybridization (FISH) demonstrates loss of 1p/1 which is also present in renal oncocytomas
Diagnostic molecular pathology, Vol.17(3), pp.141-144
09/01/2008
DOI: 10.1097/PDM.0b013e3181577d57
PMID: 18382368
Abstract
Morphologic overlap between chromophobe renal cell carcinoma (ChRCC) and renal oncocytomas (RO) has been widely recognized. Whether these tumors are genetically related and represent a spectrum of benign to malignant tumor progression remain an open question. We previously showed by conventional cytogenetics and fluorescent in situ hybridization (FISH) that the most common chromosomal abnormality in RO is loss of chromosome 1 or 1p. In this study, we evaluated chromosome 1 in ChRCC using the same set of FISH probes. Twenty-one ChRCCs from 13 men and 8 women were studied. Formalin-fixed, paraffin-embeded tissue blocks were used to construct tissue microarray. A subtelomeric 1p36.3 probe was used in tandem with 1q25 probes for FISH studies. The patients ranged in age from 34 to 82 years (mean 62.8y, median 61 y). FISH analysis showed an abnormal chromosome 1 in 20/21 (95%) ChRCCs as follows: 18 tumors (85%) had loss of entire chromosome 1.2 tumor (10%) had loss of 1p36.3 only, and 1 tumor (5%) was apparently diploid for chromosome 1. In this study, 95% of ChRCCs showed abnormality of chromosome 1 abnormalities from diploid to loss of 1p to loss of entire chromosome, is also present in oncocytomas. These results provide further evidence to support a genetic similarity between chromophobe carcinoma and oncocytoma, Whether abnormalities of chromosome 1 are associated with RO tumorigenesis or its progression to carcinoma requires further studies.
Details
- Title: Subtitle
- Chromosome 1 analysis in chromophobe renal cell carcinomas with tissue microarray (TMA)-facilitated fluorescence in situ hybridization (FISH) demonstrates loss of 1p/1 which is also present in renal oncocytomas
- Creators
- Paul N. Meyer - Loyola University Medical CenterYing Cao - University of ChicagoKris Jacobson - Vysis Abbott Mol Inc, Res & Dev, Des Plaines, IL USAThomas Krausz - University of ChicagoRobert C. Flanigan - Loyola University Medical CenterMaria M. Picken - Loyola University Medical Center
- Resource Type
- Journal article
- Publication Details
- Diagnostic molecular pathology, Vol.17(3), pp.141-144
- DOI
- 10.1097/PDM.0b013e3181577d57
- PMID
- 18382368
- NLM abbreviation
- Diagn Mol Pathol
- ISSN
- 1052-9551
- eISSN
- 1533-4066
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 4
- Language
- English
- Date published
- 09/01/2008
- Academic Unit
- Pathology
- Record Identifier
- 9984822991502771
Metrics
5 Record Views