Journal article
Chronic Low-Dose Oxidative Stress Induces Caspase-3-Dependent PKC8 Proteolytic Activation and Apoptosis in a Cell Culture Model of Dopaminergic Neurodegeneration
Annals of the New York Academy of Sciences, Vol.1139, pp.197-205
Annals of the New York Academy of Sciences
01/01/2008
DOI: 10.1196/annals.1432.020
PMCID: PMC2657189
PMID: 18991865
Abstract
Oxidative stress has been implicated as a key event in the degenerative process of dopaminergic neurons; however, the cellular mechanisms underlying chronic oxidative stress-induced neurodegeneration remain to be established. In this study, N27 cells, a dopaminergic neuronal cell line derived from rat mesencephalon, exposed to low doses of H2O2 (0-30 mu M for 12-24 hr) exhibited dose- and time-dependent increases in cytotoxicity and ROS generation. In addition, the H2O2-induced neurotoxicity was accompanied by increased caspase-3 activity and PKC delta cleavage. Notably, treatment with antioxidants Trolox and MnTBAP or PKC delta cleavage inhibitor z-DIPD-fmk significantly protected against oxidative stress-induced apoptotic cell death. These results demonstrate that the N27 cell line is a useful model for the study of the chronic low-dose oxidative stress-induced apoptotic cell death cascade and that caspase-3-dependent PKC delta proteolytic activation may be important in the apoptotic process in dopaminergic neurons undergoing chronic oxidative insult.
Details
- Title: Subtitle
- Chronic Low-Dose Oxidative Stress Induces Caspase-3-Dependent PKC8 Proteolytic Activation and Apoptosis in a Cell Culture Model of Dopaminergic Neurodegeneration
- Creators
- Martha Carvour - Iowa State UniversityChunjuan Song - Iowa State UniversitySiddharth Kaul - Iowa State UniversityVellareddy Anantharam - Iowa State UniversityAnumantha Kanthasamy - Iowa State UniversityArthi Kanthasamy
- Contributors
- M J Kuhar (Editor)
- Resource Type
- Journal article
- Publication Details
- Annals of the New York Academy of Sciences, Vol.1139, pp.197-205
- Series
- Annals of the New York Academy of Sciences
- DOI
- 10.1196/annals.1432.020
- PMID
- 18991865
- PMCID
- PMC2657189
- NLM abbreviation
- Ann N Y Acad Sci
- ISSN
- 0077-8923
- eISSN
- 1749-6632
- Publisher
- Wiley
- Number of pages
- 9
- Grant note
- NS 38644; ES 10586; RO3 NS54016 / National Institute of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA T32GM007337 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) R01NS038644 / NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Neurological Disorders & Stroke (NINDS) R01ES010586 / NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Environmental Health Sciences (NIEHS)
- Language
- English
- Date published
- 01/01/2008
- Academic Unit
- Infectious Diseases; Epidemiology; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984359957402771
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