Chronic antioxidant enzyme mimetic treatment differentially modulates hyperthermia-induced liver HSP70 expression with aging

Hannah J Zhang; Susan R Doctrow; Larry W Oberley; Kevin C Kregel

doi:10.1152/japplphysiol.01046.2005

Back

Chronic antioxidant enzyme mimetic treatment differentially modulates hyperthermia-induced liver HSP70 expression with aging

Journal article

Peer reviewed

Chronic antioxidant enzyme mimetic treatment differentially modulates hyperthermia-induced liver HSP70 expression with aging

Hannah J Zhang, Susan R Doctrow, Larry W Oberley and Kevin C Kregel

Journal of applied physiology (1985), Vol.100(4), pp.1385-1391

04/2006

DOI: 10.1152/japplphysiol.01046.2005

PMID: 16254069

View Online

Abstract

One postulated mechanism for the reduction in stress tolerance with aging is a decline in the regulation of stress-responsive genes, such as inducible heat shock protein 72 (HSP70). Increased levels of oxidative stress are also associated with aging, but it is unclear what impact a prooxidant environment might have on HSP70 gene expression. This study utilized a superoxide dismutase/catalase mimetic (Eukarion-189) to evaluate the impact of a change in redox environment on age-related HSP70 responses to a physiologically relevant heat challenge. Results demonstrate that liver HSP70 mRNA and protein levels are reduced in old compared with young rats at selected time points over a 48-h recovery period following a heat-stress protocol. While chronic systemic administration of Eukarion-189 suppressed hyperthermia-induced liver HSP70 mRNA expression in both age groups, HSP70 protein accumulation was blunted in old rats but not in their young counterparts. These data suggest that a decline in HSP70 mRNA levels may be responsible for the reduction in HSP70 protein observed in old animals after heat stress. Furthermore, improvements in redox status were associated with reduced HSP70 mRNA levels in both young and old rats, but differential effects were manifested on protein expression, suggesting that HSP70 induction is differentially regulated with aging. These findings highlight the integrated mechanisms of stress protein regulation in eukaryotic organisms responding to environmental stress, which likely involve interactions between a wide range of cellular signals.

Aging - metabolism

Animals

Antioxidants - pharmacology

Biomimetic Materials - pharmacology

Fever - metabolism

Gene Expression Regulation

HSP70 Heat-Shock Proteins - genetics

HSP70 Heat-Shock Proteins - metabolism

Liver - drug effects

Liver - metabolism

Male

Organometallic Compounds - pharmacology

Oxidation-Reduction

Rats

Rats, Inbred F344

RNA, Messenger - metabolism

Salicylates - pharmacology

Time Factors

Details

Title: Subtitle: Chronic antioxidant enzyme mimetic treatment differentially modulates hyperthermia-induced liver HSP70 expression with aging
Creators: Hannah J Zhang - Integrative Physiology Laboratory, Department of Exercise Science, 532 FH, The University of Iowa, Iowa City, 52242, USA
Susan R Doctrow
Larry W Oberley
Kevin C Kregel
Resource Type: Journal article
Publication Details: Journal of applied physiology (1985), Vol.100(4), pp.1385-1391
DOI: 10.1152/japplphysiol.01046.2005
PMID: 16254069
ISSN: 8750-7587
eISSN: 1522-1601
Grant note: AG-12350 / NIA NIH HHS CA-66081 / NCI NIH HHS
Language: English
Date published: 04/2006
Academic Unit: Radiation Oncology; Provost Office Administration; Health and Human Physiology
Record Identifier: 9984213451402771

Metrics

8 Record Views

23 Times Cited - Web of Science