Journal article
Chronic ethanol ingestion by mice increases expression of CD80 and CD86 by activated macrophages
Alcohol (Fayetteville, N.Y.), Vol.32(2), pp.91-100
2004
DOI: 10.1016/j.alcohol.2004.01.004
PMID: 15163560
Abstract
Results from previous studies from our laboratory have shown that T cells obtained from the spleens of C57BL/6 mice that consumed ethanol chronically have increased expression of activation markers and increased second signal–independent production of interferon-gamma (IFN-γ). We now report that in vitro–activated CD11b
+ splenocytes obtained from C57BL/6 and BALB/c mice that consumed ethanol chronically express increased levels of the T cell co-stimulatory molecules CD80 and CD86. CD11b
+ splenocytes encompass at least two populations: the CD11b
+Gr.1
− population, which is primarily monocytes–macrophages, and a smaller CD11b
+Gr.1
+ population, which is in the myelocytic–monocytic cell series and contains precursors of both macrophages and neutrophils. Evaluation of cultures of purified CD11b
+ cells, obtained from mice that consumed ethanol chronically, incubated overnight, showed increased up-regulation of CD80 and CD86 expression on Gr.1
− mouse splenic macrophages. Results of functional studies of purified CD11b
+ cells have demonstrated that CD11b
+ cells obtained from C57BL/6 mice that were exposed to ethanol chronically secrete higher levels, in comparison with the levels secreted by CD11b
+ cells obtained from control animals, of nitric oxide and several proinflammatory cytokines after stimulation by the oligodeoxynucleotide (ODN) CpG 1826. These findings indicate that CD11b
+ splenocytes are in some way sensitized to activating stimuli by chronic ethanol exposure in vivo. Such cells may contribute to systemic immunodysregulation, including T-cell activation, by providing abnormal second signals to T cells, or through excessive release of cytokines, such as interleukin (IL)-6 or IL-12.
Details
- Title: Subtitle
- Chronic ethanol ingestion by mice increases expression of CD80 and CD86 by activated macrophages
- Creators
- Xiaoyan Zhu - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USARuth A Coleman - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USACarol Alber - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAZuhair K Ballas - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAThomas J Waldschmidt - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USANancy B Ray - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USAArthur M Krieg - Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USARobert T Cook - Department of Pathology, Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Alcohol (Fayetteville, N.Y.), Vol.32(2), pp.91-100
- DOI
- 10.1016/j.alcohol.2004.01.004
- PMID
- 15163560
- NLM abbreviation
- Alcohol
- ISSN
- 0741-8329
- eISSN
- 1873-6823
- Publisher
- Elsevier
- Language
- English
- Date published
- 2004
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Pathology; Immunology; Internal Medicine
- Record Identifier
- 9984047893102771
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