Journal article
Chronic intermittent hypoxia alters ventilatory and metabolic responses to acute hypoxia in rats
Journal of applied physiology (1985), Vol.120(10), pp.1186-1195
05/15/2016
DOI: 10.1152/japplphysiol.00015.2016
PMCID: PMC4867317
PMID: 26917692
Abstract
We determined the effects of chronic exposure to intermittent hypoxia (CIH) on chemoreflex control of ventilation in conscious animals. Adult male Sprague-Dawley rats were exposed to CIH [nadir oxygen saturation (SpO2), 75%; 15 events/h; 10 h/day] or normoxia (NORM) for 21 days. We assessed the following responses to acute, graded hypoxia before and after exposures: ventilation (V̇e, via barometric plethysmography), V̇o2 and V̇co2 (analysis of expired air), heart rate (HR), and SpO2 (pulse oximetry via neck collar). We quantified hypoxia-induced chemoreceptor sensitivity by calculating the stimulus-response relationship between SpO2 and the ventilatory equivalent for V̇co2 (linear regression). An additional aim was to determine whether CIH causes proliferation of carotid body glomus cells (using bromodeoxyuridine). CIH exposure increased the slope of the V̇e/V̇co2/SpO2 relationship and caused hyperventilation in normoxia. Bromodeoxyuridine staining was comparable in CIH and NORM. Thus our CIH paradigm augmented hypoxic chemosensitivity without causing glomus cell proliferation.
Details
- Title: Subtitle
- Chronic intermittent hypoxia alters ventilatory and metabolic responses to acute hypoxia in rats
- Creators
- Barbara J Morgan - John Rankin Laboratory of Pulmonary Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin; Department of Orthopedics and Rehabilitation, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin; bjmorgan@wisc.eduRussell Adrian - John Rankin Laboratory of Pulmonary Medicine, School of Medicine and Public Health, University of Wisconsin, Madison, Wisconsin; Department of Orthopedics and Rehabilitation, School of Medicine and Public Health, University of Wisconsin, Madison, WisconsinZun-Yi Wang - Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WisconsinMelissa L Bates - Department of Health and Human Physiology, College of Liberal Arts and Sciences, University of Iowa, Iowa City, Iowa; andJohn M Dopp - Pharmacy Practice Division, School of Pharmacy, University of Wisconsin, Madison, Wisconsin
- Resource Type
- Journal article
- Publication Details
- Journal of applied physiology (1985), Vol.120(10), pp.1186-1195
- Publisher
- United States
- DOI
- 10.1152/japplphysiol.00015.2016
- PMID
- 26917692
- PMCID
- PMC4867317
- ISSN
- 8750-7587
- eISSN
- 1522-1601
- Grant note
- U01 HL105365 / NHLBI NIH HHS P30 CA014520 / NCI NIH HHS
- Language
- English
- Date published
- 05/15/2016
- Academic Unit
- Stead Family Department of Pediatrics; Health and Human Physiology; Internal Medicine
- Record Identifier
- 9984002336102771
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