Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation

Zhenhua Ren; Fanmuyi Yang; Xin Wang; Yongchao Wang; Mei Xu; Jacqueline A Frank; Zun-ji Ke; Zhuo Zhang; Xianglin Shi; Jia Luo

doi:10.1016/j.taap.2016.08.012

Back

Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation

Journal article

Open access

Peer reviewed

Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation

Zhenhua Ren, Fanmuyi Yang, Xin Wang, Yongchao Wang, Mei Xu, Jacqueline A Frank, Zun-ji Ke, Zhuo Zhang, Xianglin Shi and Jia Luo

Toxicology and applied pharmacology, Vol.308, pp.11-19

10/01/2016

DOI: 10.1016/j.taap.2016.08.012

PMCID: PMC5023495

PMID: 27538709

Files and links (1)

url

https://doi.org/10.1016/j.taap.2016.08.012View

Published (Version of record) Open Access

Abstract

Alcohol abuse increases the risk for pancreatitis. The pattern of alcohol drinking may impact its effect. We tested a hypothesis that chronic ethanol consumption in combination with binge exposure imposes more severe damage to the pancreas. C57BL/6 mice were divided into four groups: control, chronic ethanol exposure, binge ethanol exposure and chronic plus binge ethanol exposure. For the control group, mice were fed with a liquid diet for two weeks. For the chronic ethanol exposure group, mice were fed with a liquid diet containing 5% ethanol for two weeks. In the binge ethanol exposure group, mice were treated with ethanol by gavage (5g/kg, 25% ethanol w/v) daily for 3days. For the chronic plus binge exposure group, mice were fed with a liquid diet containing 5% ethanol for two weeks and exposed to ethanol by gavage during the last 3days. Chronic and binge exposure alone caused minimal pancreatic injury. However, chronic plus binge ethanol exposure induced significant apoptotic cell death. Chronic plus binge ethanol exposure altered the levels of alpha-amylase, glucose and insulin. Chronic plus binge ethanol exposure caused pancreatic inflammation which was shown by the macrophages infiltration and the increase of cytokines and chemokines. Chronic plus binge ethanol exposure increased the expression of ADH1 and CYP2E1. It also induced endoplasmic reticulum stress which was demonstrated by the unfolded protein response. In addition, chronic plus binge ethanol exposure increased protein oxidation and lipid peroxidation, indicating oxidative stress. Therefore, chronic plus binge ethanol exposure is more detrimental to the pancreas. •Chronic plus binge alcohol drinking causes more pancreatic injury.•Chronic plus binge alcohol drinking induces more pancreatic inflammation.•Chronic plus binge alcohol causes more endoplasmic reticulum stress and oxidative stress.

Alcohol

Apoptosis

Drinking pattern

Inflammation

Insulin

Pancreatitis

Details

Title: Subtitle: Chronic plus binge ethanol exposure causes more severe pancreatic injury and inflammation
Creators: Zhenhua Ren - University of Kentucky
Fanmuyi Yang - University of Kentucky
Xin Wang - University of Kentucky
Yongchao Wang - University of Kentucky
Mei Xu - University of Kentucky
Jacqueline A Frank - University of Kentucky
Zun-ji Ke - Shanghai University of Traditional Chinese Medicine
Zhuo Zhang - University of Kentucky
Xianglin Shi - University of Kentucky
Jia Luo - University of Kentucky
Resource Type: Journal article
Publication Details: Toxicology and applied pharmacology, Vol.308, pp.11-19
DOI: 10.1016/j.taap.2016.08.012
PMID: 27538709
PMCID: PMC5023495
NLM abbreviation: Toxicol Appl Pharmacol
ISSN: 0041-008X
eISSN: 1096-0333
Publisher: Elsevier Inc
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: AA017226, AA015407; DOI: 10.13039/100000738, name: U.S. Department of Veterans Affairs, award: BX001721
Language: English
Date published: 10/01/2016
Academic Unit: Pathology
Record Identifier: 9984201124002771

Metrics

13 Record Views

19 Times Cited - Web of Science