Journal article
Chronic pulmonary accumulation of iron oxide nanoparticles induced Th1-type immune response stimulating the function of antigen-presenting cells
Environmental research, Vol.143(Pt A), pp.138-147
11/2015
DOI: 10.1016/j.envres.2015.09.030
PMID: 26492398
Abstract
Although there is growing evidence that suggests that pulmonary exposure to nanoparticles causes adverse health effects by modulating immune system of the body, available information is very limited. In this study, we investigated immune response following chronic pulmonary accumulation of iron oxide nanoparticles (FeNPs, Fe2O3). FeNPs have a needle-like shape in suspension (101.3±4.2nm). On day 90 after a single intratracheal instillation (0.5, 1, and 2mg/kg), the FeNPs remained in the lung and particle-laden macrophages were clearly observed in the BAL fluid of the treated-mice. The number of total cells and proportions of neutrophils and lymphocytes significantly increased at 2mg/kg dose, and the percentage of apoptotic cells and LDH release increased in a dose-dependent manner. We also found that Th1-polarized inflammatory response was induced in the lung of the treated group accompanying the elevated secretion of chemokines, including GM-CSF, MCP-1, and MIP-1. Additionally, FeNPs enhanced the expression of antigen presentation-related proteins, including CD80, CD86, and MHC class II, on antigen-presenting cells in BAL fluid. Taken together, we suggest that chronic pulmonary accumulation of FeNPs may induce Th1-polarized immune response augmenting the function of antigen-presenting cells in the lung.
•We identified immunotoxicity following chronic pulmonary accumulation of FeNPs.•FeNPs remained in the lung until 90 days after a single intratracheal instillation.•FeNPs induced Th1-polarized immune response with the elevated secretion of chemokines.•FeNPs enhanced the function of antigen-presenting cells (APCs) in the lung.•FeNPs systemically attenuated the function of antigen-presenting cells (APCs).
Details
- Title: Subtitle
- Chronic pulmonary accumulation of iron oxide nanoparticles induced Th1-type immune response stimulating the function of antigen-presenting cells
- Creators
- Eun-Jung Park - Myunggok Eye Research Institute, Konyang University, 685, Gasuwon-dong, Seo-Gu, Daejeon 302-718, Republic of KoreaSeung Yun Oh - Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Republic of KoreaSang Jin Lee - Inhalation Toxicology Center, Korea Institute of Toxicology, Jeongeup 580-185, Republic of KoreaKyuhong Lee - Inhalation Toxicology Center, Korea Institute of Toxicology, Jeongeup 580-185, Republic of KoreaYounghun Kim - Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Republic of KoreaByoung-Seok Lee - Toxicologic Pathology Center, Korea Institute of Toxicology, Daejeon, Republic of KoreaJong Sung Kim - Department of Community Health and Epidemiology, Faculty of Medicine, Dalhousie University, Halifax, Canada
- Resource Type
- Journal article
- Publication Details
- Environmental research, Vol.143(Pt A), pp.138-147
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.envres.2015.09.030
- PMID
- 26492398
- ISSN
- 0013-9351
- eISSN
- 1096-0953
- Grant note
- DOI: 10.13039/501100004085, name: Ministry of Education, Science and Technology, award: 2011-35B-E00011; DOI: 10.13039/501100003725, name: National Research Foundation of Korea
- Language
- English
- Date published
- 11/2015
- Academic Unit
- Occupational and Environmental Health
- Record Identifier
- 9984214941302771
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