Cigarette Smoke Alters the Hematopoietic Stem Cell Niche

Robert W Siggins; Fokhrul Hossain; Tayyab Rehman; John N Melvan; Ping Zhang; David A Welsh

doi:10.3390/medsci2010037

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Cigarette Smoke Alters the Hematopoietic Stem Cell Niche

Journal article

Open access

Peer reviewed

Cigarette Smoke Alters the Hematopoietic Stem Cell Niche

Robert W Siggins, Fokhrul Hossain, Tayyab Rehman, John N Melvan, Ping Zhang and David A Welsh

Medical sciences (Basel), Vol.2(1), pp.37-50

03/2014

DOI: 10.3390/medsci2010037

PMCID: PMC5576506

PMID: 28868162

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Abstract

Effects of tobacco smoke on hematologic derangements have received little attention. This study employed a mouse model of cigarette smoke exposure to explore the effects on bone marrow niche function. While lung cancer is the most widely studied consequence of tobacco smoke exposure, other malignancies, including leukemia, are associated with tobacco smoke exposure. Animals received cigarette smoke exposure for 6 h/day, 5 days/week for 9 months. Results reveal that the hematopoietic stem and progenitor cell (HSPC) pool size is reduced by cigarette smoke exposure. We next examined the effect of cigarette smoke exposure on one supporting cell type of the niche, the mesenchymal stromal cells (MSCs). Smoke exposure decreased the number of MSCs. Transplantation of naïve HSPCs into irradiated mice with cigarette smoke exposure yielded fewer numbers of engrafted HSPCs. This result suggests that smoke-exposed mice possess dysfunctional niches, resulting in abnormal hematopoiesis. Co-culture experiments using MSCs isolated from control or cigarette smoke-exposed mice with naïve HSPCs showed that MSCs from cigarette smoke-exposed mice generated marked expansion of naïve HSPCs. These data show that cigarette smoke exposure decreases MSC and HSC number and also increases pro-proliferative gene expression by cigarette smoke-exposed MSCs, which may stimulate HSPC expansion. These results of this investigation are clinically relevant to both bone marrow donors with a history of smoking and bone marrow transplant (BMT) recipients with a history of smoking.

hematopoietic stem cell

hematopoiesis

niche

mesenchymal stromal cell

cigarette smoke

Details

Title: Subtitle: Cigarette Smoke Alters the Hematopoietic Stem Cell Niche
Creators: Robert W Siggins - Alcohol and Drug Abuse Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
Fokhrul Hossain - Department of Biochemistry and Molecular Biology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
Tayyab Rehman - Department of Medicine, Section of Pulmonary and Critical Care Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
John N Melvan - Department of Physiology, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
Ping Zhang - Department of Surgery, Michigan State University, East Lansing, MI 48824, USA
David A Welsh - Department of Medicine, Section of Pulmonary and Critical Care Medicine, Louisiana State University Health Sciences Center, New Orleans, LA 70112, USA
Resource Type: Journal article
Publication Details: Medical sciences (Basel), Vol.2(1), pp.37-50
DOI: 10.3390/medsci2010037
PMID: 28868162
PMCID: PMC5576506
NLM abbreviation: Med Sci (Basel)
ISSN: 2076-3271
eISSN: 2076-3271
Grant note: R01 AA019676 / NIAAA NIH HHS K99 AA020312 / NIAAA NIH HHS R00 AA020312 / NIAAA NIH HHS R01 HL073770 / NHLBI NIH HHS P50 AA009803 / NIAAA NIH HHS
Language: English
Date published: 03/2014
Academic Unit: Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
Record Identifier: 9984094358402771

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