Journal article
Cigarette Smoke Induces Cellular Senescence
American journal of respiratory cell and molecular biology, Vol.35(6), pp.681-688
12/2006
DOI: 10.1165/rcmb.2006-0169OC
PMCID: PMC2643295
PMID: 16840774
Abstract
Chronic obstructive pulmonary disease (COPD) is the fourth leading cause of death in the United States, and cigarette smoking is the major risk factor for COPD. Fibroblasts play an important role in repair and lung homeostasis. Recent studies have demonstrated a reduced growth rate for lung fibroblasts in patients with COPD. In this study we examined the effect of cigarette smoke extract (CSE) on fibroblast proliferative capacity. We found that cigarette smoke stopped proliferation of lung fibroblasts and upregulated two pathways linked to cell senescence (a biological process associated with cell longevity and an inability to replicate), p53 and p16-retinoblastoma protein pathways. We compared a single exposure of CSE to multiple exposures over an extended time course. A single exposure to CSE led to cell growth inhibition at multiple phases of the cell cycle without killing the cells. The decrease in proliferation was accompanied by increased ATM, p53, and p21 activity. However, several important senescent markers were not present in the cells at an earlier time point. When we examined multiple exposures to CSE, we found that the cells had profound growth arrest, a flat and enlarged morphology, upregulated p16, and senescence-associated β-galactosidase activity, which is consistent with a classic senescent phenotype. These observations suggest that while a single exposure to cigarette smoke inhibits normal fibroblast proliferation (required for lung repair), multiple exposures to cigarette smoke move cells into an irreversible state of senescence. This inability to repair lung injury may be an essential feature of emphysema.
Details
- Title: Subtitle
- Cigarette Smoke Induces Cellular Senescence
- Creators
- Toru Nyunoya - Division of Pulmonary, Critical Care, and Occupational Medicine, and the Department of Microbiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine; and Veterans Administration Medical Center, Iowa City, IowaMartha M Monick - Division of Pulmonary, Critical Care, and Occupational Medicine, and the Department of Microbiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine; and Veterans Administration Medical Center, Iowa City, IowaAloysius Klingelhutz - Division of Pulmonary, Critical Care, and Occupational Medicine, and the Department of Microbiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine; and Veterans Administration Medical Center, Iowa City, IowaTimur O Yarovinsky - Division of Pulmonary, Critical Care, and Occupational Medicine, and the Department of Microbiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine; and Veterans Administration Medical Center, Iowa City, IowaJeffrey R Cagley - Division of Pulmonary, Critical Care, and Occupational Medicine, and the Department of Microbiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine; and Veterans Administration Medical Center, Iowa City, IowaGary W Hunninghake - Division of Pulmonary, Critical Care, and Occupational Medicine, and the Department of Microbiology, University of Iowa Roy J. and Lucille A. Carver College of Medicine; and Veterans Administration Medical Center, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- American journal of respiratory cell and molecular biology, Vol.35(6), pp.681-688
- DOI
- 10.1165/rcmb.2006-0169OC
- PMID
- 16840774
- PMCID
- PMC2643295
- NLM abbreviation
- Am J Respir Cell Mol Biol
- ISSN
- 1044-1549
- eISSN
- 1535-4989
- Publisher
- American Thoracic Society
- Language
- English
- Date published
- 12/2006
- Academic Unit
- Microbiology and Immunology; Radiation Oncology; Internal Medicine
- Record Identifier
- 9984001209502771
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