Journal article
Cilia containing 9 + 2 structures grown from immortalized cells
Cell research, Vol.17(6), pp.537-545
06/2007
DOI: 10.1038/sj.cr.7310151
PMID: 17438554
Abstract
Cilia depend on their highly differentiated structure, a 9 + 2 arrangement, to remove particles from the lung and to transport reproductive cells. Immortalized cells could potentially be of great use in cilia research. Immortalization of cells with cilia structure containing the 9 + 2 arrangement might be able to generate cell lines with such cilia structure. However, whether immortalized cells can retain such a highly differentiated structure remains unclear. Here we demonstrate that (1) using E1a gene transfection, tracheal cells are immortalized; (2) interestingly, in a gel culture the immortalized cells form spherical aggregations within which a lumen is developed; and (3) surprisingly, inside the aggregation, cilia containing a 9 + 2 arrangement grow from the cell's apical pole and protrude into the lumen. These results may influence future research in many areas such as understanding the mechanisms of cilia differentiation, cilia generation in other existing cell lines, cilia disorders, generation of other highly differentiated structures besides cilia using the gel culture, immortalization of other ciliated cells with the E1a gene, development of cilia motile function, and establishment of a research model to provide uniform ciliated cells.
Details
- Title: Subtitle
- Cilia containing 9 + 2 structures grown from immortalized cells
- Creators
- Ming Zhang - Department of Speech Language and Hearing Sciences, Texas Tech University - Health Sciences Center, Lubbock, TX 79430, USA. Ming.zhang@ttuhsc.eduJose G Assouline
- Resource Type
- Journal article
- Publication Details
- Cell research, Vol.17(6), pp.537-545
- Publisher
- England
- DOI
- 10.1038/sj.cr.7310151
- PMID
- 17438554
- ISSN
- 1001-0602
- eISSN
- 1748-7838
- Language
- English
- Date published
- 06/2007
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Urology
- Record Identifier
- 9984070163002771
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